Hello Bio/D-AP5/HB0225/50mg

Name
D-AP5
Shortdescription
Selective,competitiveNMDAreceptorantagoNIST
BIOLOGicaldescription
Widelyused,selectiveandcompetitiveNMDAreceptorantagonistwhichbindsatglutamatesite.MoreactiveformofDL-AP5.BlocksinductionofLTP(longtermpotentiation)andimpairsspatiallearning.
Alternativenames
2-APV, D-APV
Biologicalaction
Antagonist
Purity
>99%
Customercomments
ImadethediscoverythattheNMDAreceptoristhetriggerfortheinductionofLTPusingD-AP5synthesizedbyJeffWatkins,thediscovereroftheNMDAreceptor...InowobtainmyD-AP5fromHelloBio.IlovetheirproductsandethosandthatiswhyIacceptedapositionontheirScientificAdvisoryBoard.
ProfessorGrahamCollingridge,winnerofTheBrainPrize,2016
MylabusedD-AP5fromHelloBioandwereveryhappywithit.Itbehavedexactlyasexpected!ProfessorKeiCho,ChairofNeuroscience,UniversityofBristol,UK(HelloBioScientificAdvisoryBoardMember)
MylabisverysatisfiedwithyourD-AP5qualityandprice.Verifiedcustomer,EuropeanBrainResearchInstitute(EBRI)
Ourproductsinaction
7citationsSubmitYourCitationNow

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Chemicalname
D-(-)-2-Amino-5-phosphonopentanoicacid
MolecularWeight
197.13
Chemicalstructure
MolecularFormula
C₅H₁₂NO₅P
CASNumber
79055-68-8
PubChemidentifier
135342
SMILES
N[C@H](CCCP(=O)(O)O)C(=O)O
Source
Synthetic
InChi
InChI=1S/C5H12NO5P/c6-4(5(7)8)2-1-3-12(9,10)11/h4H,1-3,6H2,(H,7,8)(H2,9,10,11)/t4-/m1/s1
InChiKey
VOROEQBFPPIACJ-SCSAIBSYSA-N
MDLnumber
MFCD00078839
Appearance
Whitesolid

Applicationnotes
TheNMDAreceptorantagonistD-AP5iscommonlyusedtoinhibitNMDAmediatedsynapticplasticity.Itisoftenusedatconcentrationsof50μM.D-AP5fromHelloBiocompletelyabolishesevokedNMDARmediatedcurrentsat50μMandreducesNMDAcurrentsatlowerconcentrationsof1and10μM(seeFig1above).

#Protocol1:EvokedNMDAreceptorcurrents
- WholecellvoltageclamprecordingswereobtainedfromlayerVneuronsofthemouseprelimbiccortexbrainslice.
- NMDAcurrentswereevokedviaastimulatingelectrodeplacedinlayersII/IIIandevokedbyasinglesquare(150μs)pulseevery10secatastimulusintensitythatgaveareliableNMDAcurrent.
- Neuronswerehelda+40mVtorelieveNMDAcurrentsfromtheirvoltage-dependentMg²⁺block.
- NMDAcurrentswerecontinuallystimulatedandrecordedinresponsetocontinualbathapplicationsofD-AP5untilNMDAcurrentswerecompletelyabolished.
- AllNMDARrecordingsweremadeinthepresenceofGABA_A-RandAMPARantagonists.

Storageinstructions
Roomtemperature
Solubilityoverview
Solubleinwater(100mM)
Important
ThisproductisforRESEARCHUSEONLYandisnotintendedfortherapeuticordiagnosticuse.Notforhumanorveterinaryuse.

NMDAreceptors,learningandmemory:chronicintraventricularinfusionoftheNMDAreceptorantagonistd-AP5interactsdirectlywiththeneuralmechanismsofspatiallearning.
MorrisRGetal(2013)EurJNeurosci37(5):700-17.
PubMedID:23311352
ActionsofDandLformsof2-amino-5-phosphonovalerateand2-amino-4-phosphonobutyrateinthecatspinalcord.
DaviesJetal(1982)BrainRes235(2):378-86.
PubMedID:6145492
EffectsofpreorposttrainingdorsalhippocampusD-AP5injectiononfearconditioningtotone,background,andforegroundcontext.
SchenbergEEetal(2008)Hippocampus18(11):1089-93.
PubMedID:18727044