企业QQ
官方微信
Theantibodyisprovidedas100uLofaffinitypurifiedseruminPBS(0.02Msodiumphosphatewith0.15Msodiumchloride,pH7.5)with1%BSA(bovineserumalbumin),and0.02%sodiumazide. Reactswithmouse,rat,andrabbitspecies.
Theimagesabovelabeled"ImmunoStarRabbit5-HT2Areceptorantibody"aretheresultsofstainingofthemouse5-HT2Areceptorinthebrain,courtesyofDr.MagdalenaZaniewska,Max-Delbruck-CentrumfurMolekulareMedizinBerlin,Germany.
TheImmunoStar5HT2Areceptorantibodywasqualitycontroltestedusingstandardimmunohistochemicalmethods.Theantiserumdemonstratesstronglypositivelabelingofratcortex,amygdalaandhippocampususingindirectimmunofluorescentandbiotin/avidin-HRPtechniques.Recommendedprimarydilutionsare1/300-1/500inPBS/0.3%TritonX-100 -Bn/Av-HRPTechnique.Theadditionofintensifyingreagentssuchasnickelammoniumsulfatetothechromogensolutionwillapproximatelydoublethedilutionfactorasrecommended.
Immunolabelingiscompletelyabolishedbypreadsorptionwithsyntheticrat5HT2Areceptor(22-41).ImmunolabelingofWesternblotrevealedasinglebandofapproximately53kD.
PhotoDescription: LowmagnificationIHCimageofneuronsstainingforthe5-HT2Areceptorintheratcortex(topofpage)andimageofneuronalexpressionofthereceptorintheamygdala(below).Thebottomrightphotoisofthecortex. Thetissuewasfixedwith4%formaldehydein0.1Mphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:500for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host:Rabbit
Quantity/Volume:100uL
State:Liquid
ReactsWith:Human,Marine,Mouse,Rabbit,Rat
AlternateNames: 5-hydroxytryptaminereceptor2A;5Ht-2;5-hydroxytryptamine(SEROtonin)receptor2A,Gprotein-coupled,anti-5-HT2A
GeneSymbol/ID,Accession#: Htr2a,29595
RRID: AB_572211
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn