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TheImmunoStar5-HT7receptorantiserumwasqualitycontroltestedusingstandardimmunohistochemicalmethods.Theantiserumdemonstratesstronglypositivelabelingofratcortexandhippocampususingindirectimmunofluorescentandbiotin/avidin-HRPtechniques.Recommendedprimarydilutionis1/100-1/300inPBS-biotin/avidin-HRPTechnique.Note:useofTritonX-100orotherdetergentsisnotrecommended.
Theantibodywascharacterizedbyimmunohistochemistry.ImmunohistochemicalstainingofratbraincorrelateswellwithNorthernblotanalysis,insituhybridizationandreceptorautorADIographystudies.Immunolabelingiscompletelyabolishedbypreadsorptionwithsyntheticrat5-HT7receptor(8-23).BlastPdatabasesequencehomologysearchesindicatethattheaminoacidsequenceisuniquetorat5-HT7A,5-HT7Band5-HT7C.Thereisalsosignificantsequenceoverlapwiththemouseandhumanforms.
PhotoDescription:IHCimageofneuronsstainingforthe5-HT7receptorintheratbraincortex.Thetissuewasfixedwith4%formaldehyde/0.05%glutaraldehydein0.1Mphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:500for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host:Rabbit
Quantity/Volume:100µL
State:Liquid
ReactsWith:Cat,Grasshopper,Human,HutchinsoniellaMacracantha(HorseshoeShrimp),Mouse,Pig,Rabbit,Rat
AvailABIlity: Instock
AlternateNames: 5-hydroxytryptaminereceptor7;5-HT-X,GPRFO;5Ht7;5-hydroxytryptamine(SEROtonin)receptor7,anti-5-HT7
RRID:AB_572214
Immunogen:Rat5-HT7receptor(8-23)
GeneSymbol/ID,Accession#: Htr7,65032
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn