Theantibodyhasaproven maximumbiotin-streptavidin/HRPstainingata1/1000-1/2000dilutioninratraphenuclei.Optimaldilutionwillvarydependinguponfixation,labelingtechniqueand/ordetectionsystem;therefore,adilutionseriesisrecommended.
Thespecificityoftheantiserumwasevaluatedusingamodelsystemofgelatin-indoleplugsbyamethodsimilartopublishedprocedures(ShipperandTilders,1983).Resultsshowedthatthe5-HTPantibodydosedependentlystained5-HTPbutdidnotstainanyconcentrationof5-HTor5-HIAA.
Theantiserumwasalsotestedbypre-adsorptionwithindole/paraformaldehyde/BSAconjugates.Stainingwascompletelyblockedbypre-adsorptionwith5-HTPconjugateandunaffectedby5-HIAAor5-HTconjugate.
PhotoDescription:IHCimageofneuronsstainingfor5-HTPintheraphenucleusoftheratbrainstem.Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:2000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host:Rabbit
Quantity/Volume:100µL
State:LyophilizedWholeSerum
ReactsWith:Rat,SeaSlug(AplysiaCalifornica,TritoniaDiomedea
AvailABIlity:InStock
AlternateNames: anti-5-HTP
RRID: AB_572215
Immunogen:5-HTP
GeneSymbol:Avp
EntrezGeneID:24221
NCBIGeneAliases:DI,Va
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn