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Theantibodyproducesa maximumbiotin-streptavidin/HRPstainingata1/500-1/1000dilutioninratanterior/intermediatepituitary.Optimaldilutionwillvarydependinguponfixation,labelingtechniqueand/ordetectionsystem;therefore,adilutionseriesisrecommended.Therecommeneddilutionseriesis1/500-1/1000inPBS/0.3%TritonX-100-Bn-SA/HRPTechnique.Stainingiscompletelyeliminatedbypretreatmentofthedilutedantibodywith100µg/mLofACTH.
PhotoDescription:IHCimageofneuronsstainingforACTHintheratanteriorpituitarygland.Thetissuewasfixedwith4%formaldehydein0.1Mphosphatebuffer,beforebeingremovedandpreparedforfrozensectioning.SectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:1000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host:Rabbit
Quantity/Volume:100µL
State:LyophilizedWholeSerum
AvailABIlity:InStock
ReactsWith:Dove,Eel,Goldfish,Hamster,Human,Monkey,Mouse,Ram(Sheep),Rat,Trout
AlternateNames: anti-ACTH
RRID: AB_572216
Immunogen:AdrenocorticotropicHormone
GeneSymbol/ID,Accession#: POMC,396863
NCBIGeneAliases:ACTHR,MGC12579
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