Theantibodyhasaproven maximumbiotin-avidin/HRPstainingata1/1000-1/4000dilutioninratcortex,hippocampusandhypothalamus.
Theantiserumhasbeencharacterizedasspecifictocalretinin;pleaseseereferenceslistedbelow.Optimaldilutionwillvarydependinguponfixation,labelingtechniqueand/ordetectionsystem;therefore,adilutionseriesisrecommended
PhotoDescription: IHCimageofneuronsstainingforcalretininintheratcortex(above)andtheratdentategyrus(below).Thetissuewasfixedwith4%formaldehydein0.1Mphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:4000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host: Rabbit
Quantity/Volume: 100µL
State:LyophilizedWholeSerum
ReactsWith:Rat
AvailABIlity:InStock
RRID: AB_572223
Immunogen:ChickCalretininfusionprotein
GeneSymbol: CALB2
EntrezGeneID:794
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn