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ImmunoStar/Calretinin Antibody/24445/1 Ea

Description

Theantibodyhasaproven maximumbiotin-avidin/HRPstainingata1/1000-1/4000dilutioninratcortex,hippocampusandhypothalamus.

Theantiserumhasbeencharacterizedasspecifictocalretinin;pleaseseereferenceslistedbelow.Optimaldilutionwillvarydependinguponfixation,labelingtechniqueand/ordetectionsystem;therefore,adilutionseriesisrecommended

PhotoDescription: IHCimageofneuronsstainingforcalretininintheratcortex(above)andtheratdentategyrus(below).Thetissuewasfixedwith4%formaldehydein0.1Mphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:4000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.  

Calretinin dentate gyrus #24445

Host: Rabbit

Quantity/Volume: 100µL

State:LyophilizedWholeSerum

ReactsWith:Rat

AvailABIlity:InStock

RRID: AB_572223

Immunogen:
ChickCalretininfusionprotein

GeneSymbol: 
CALB2

EntrezGeneID:
794

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