ImmunoStar/FMRF-amide (Cardio-excitatory Peptide) Antibody/20091/1 Ea

TheImmunoStarFMRF-Amideantiserumwasqualitycontroltestedusingstandardimmunohistochemicalmethods.Theantiserumdemonstratesstronglypositivelabelingofrathypothalamusandspinalcordusingindirectimmunofluorescentandbiotin/avidin-HRPtechniques. Recommendedprimarydilutionis1/1,000-1/2,000inPBS/0.3%TritonX-100-biotin/avidin-HRPTechnique.

Stainingiscompletelyeliminatedbypretreatmentofthedilutedantibodywith100µg/mLofFMRF-Amide.

PhotoDescription:IHCimagesofFMRF-amidestainingintherathypothalamuswithnickelpreparatio(above)and inthedorsalhornoftheratspinalcordwithstandardbiotin-streptavidin/HRPandDABchromogen(below).Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioningforthehypothalamus,andfrozensectionforthespinalcord.SectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:2000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.

Host: Rabbit

Quantity/Volume: 100µL

State:LyophilizedWholeSerum

ReactsWith:Abalone(SeaSnail),Acoela(Flatworm),Annelida(SegmentedWorm),Anthomedusae,Aplysia(SeaSlug),AplysiaCalifornica(SeaSlug),Bat,Beetle,Brachiopods,BrownTrout(Fish),Bullfrog,Caenogastropod,Caenogastropod,CarausiusMorosus(Insect),Carp(Fish),Catfish,Centipede,Chiton,CichlidFish,ClioneLimacinaPteropod(FruitBat),Cockroach,Crab,Crayfish,Crocodile,Crustacean,DecapodCrustacea,DermophisMexicanusMexicanCaecilian (Amphibian),DiplopteraPunctata(BeetleCockroach),Earwig/forcepbug,Fish,Fly,Frog(XenopusLaevis),Frog(XenopusTectum),Gastropod(Slug),Gerbil,Hagfish,Hagfish,HelixPomatia(Snail),HermitCrab,Human,IdoteaEmarginata(Isopod),IlyanassaObsoleta(SeaSnail),Jellyfish,Lamprey,Leech,Lepidodasy,LepismachilisY-Signata,Lizard,Lobster,Locust,LocustaMigratoria(Locust),LymnaeaStagnalis(Snail),Macrobiotus(WaterBear),ManducaSexta(Moth),Marine,MarineGastrotrichs,Micro-Organisms,Mollusc,Mollusca,Mollusk,Monkey,MopaliaMuscosa(MossyChiton),Moth,Mouse,Mussel,Mussel(MytilusTrossulus),Octocorallia(SoftCoral),PhascolionStrombus(Sipuncula),PhestillaSibogae(SeaSlug),PhyllodoceMaculata(Phyllodocidae),Planarian,PleurobranchaeaJaponica(SeaSlug),Polyplacophora(Chitons),PseudaletiaUnipuncta(Moth),PygmySquid,Ragworm,Rat,Ray,RhodniusProlixus(Bug),Salamander(Urodele),SeaCucumber,SeaPansy,SeaSlug,Shark,Shrew,Skark,Slug,Snail,SpadellaCephaloptera(ArrowWorm),SpionidPolychaete,Three-SpinedStickleback(Fish),Tick,Trout,Worm(Digenea),Worm(EiseniaFetida),Worm(LumbricusTerrestris),Zebrafish

AvailABIlity:InStock

AlternateNames:Anti-FMRF

RRID: AB_572232

Immunogen:FMRF-Amide

GeneSymbol:LOC100533232

EntrezGeneID:100533232