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ImmunoStar/Glucagon Antibody/20076/1 Ea

Description

TheantibodyhasaprovenstrongBiotin-Streptavidin/HRPimmunostainingata1/500-1/1000dilutioninhumanpancreaticislets.Stainingiscompletelyeliminatedbypretreatmentofthedilutedantibodyinanexcessofglucagon.

Preadsorptionofthedilutedantibodywithanexcessofthefollowingsubstanceshadnoeffectonglucagonlabeling:secretin,vasoactiveintestinalpeptide,peptidehistidineisoleucine-27,gastricinhibitorypolypeptide,ratandhumangrowthhormonereleasinghormoneandsomatostatin.

PhotoDescription:IHCimageofalphacellsintheisletsofLangerhansoftheratpancreasstainingforglucagon(above)andcounterstainedwithhemotoxylin(below).Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforfrozensectioning.SectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:1000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen. 

Glucagon counterstained #20076

Host: Rabbit

Quantity/Volume: 100µL

State:LyophilizedWholeSerum

ReactsWith:Chick,Human,Mouse,Pig,Rat,SeaLamprey,Snail,Three-SpinedStickleback(Fish),Turtle

AvailABIlity:InStock

AlternateNames: anti-glucagon

GeneSymbol/ID,Accession#: GCG,2641,280802,397595

 RRID: AB_572241

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