TheantibodyhasaprovenstrongBiotin-Streptavidin/HRPimmunostainingata1/500-1/1000dilutioninhumanpancreaticislets.Stainingiscompletelyeliminatedbypretreatmentofthedilutedantibodyinanexcessofglucagon.
Preadsorptionofthedilutedantibodywithanexcessofthefollowingsubstanceshadnoeffectonglucagonlabeling:secretin,vasoactiveintestinalpeptide,peptidehistidineisoleucine-27,gastricinhibitorypolypeptide,ratandhumangrowthhormonereleasinghormoneandsomatostatin.
PhotoDescription:IHCimageofalphacellsintheisletsofLangerhansoftheratpancreasstainingforglucagon(above)andcounterstainedwithhemotoxylin(below).Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforfrozensectioning.SectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:1000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host: Rabbit
Quantity/Volume: 100µL
State:LyophilizedWholeSerum
ReactsWith:Chick,Human,Mouse,Pig,Rat,SeaLamprey,Snail,Three-SpinedStickleback(Fish),Turtle
AvailABIlity:InStock
AlternateNames: anti-glucagon
GeneSymbol/ID,Accession#: GCG,2641,280802,397595
RRID: AB_572241
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn