企业QQ
官方微信
TheantibodyproducesstronglabelingofGluR1atdilutionsof1/4,000-1/6,000usingbiotin-streptavidinperoxidasetechniqueinratcortexandhippocampus.Optimaldilutionwillvarydependinguponfixation,labelingtechniqueand/ordetectionsystem;therefore,adilutionseriesisrecommended.
WesternblotanalysisofGluR1transfectedcellsandratbrainhomogenatestheantibodyspecificallylabelsasinglebandatapproximately102kD.WesternblotanalysisofGluR2,3,4,4C,5,6,and7transfectedcellsrevealednoimmunolabeling.Immunolabelingoftheabovenon-NMDAtransfectedcellsdemonstratesspecificityforGluR1.Additionally,immunolabelingforGluR1iscompletelyabolishedbypre-adsorptionwithsyntheticratGluR1(894-907)at5µgpermLofdilutedantibody.
PhotoDescription:IHCimageofneuronsstainingforGluR1intheratcortex(above)andinalowmagnificationimageoftherathippocampus(below).Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:5000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host:Rabbit
Quantity/Volume:100µL
State:Lyophilized
ReactsWith:Rat
AlternateNames: AMPA-selectiveglutamatereceptor1;AMPA1;GLUH1;GluR-A;GluR-K1;Glutamatereceptorionotropic,AMPA1;Glutamatereceptorionotropic,kainate1;GluK1;GluA1;glutamatereceptor,ionotropic,AMPA1(alpha1),anti-GluR1
GeneSymbol/ID,Accession#: Gria1,50592
RRID: AB_572243
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn