TheImmunoStarNK1Rantiserumwasqualitycontroltestedusingstandardimmunohistochemicalmethodsinratbrainusingbiotin/avidin-HRPtechniques.Specificityoftheantiserumwasdemonstratedbysolublepre-adsorptionandWesternblot.Tissuestainingiscompletelyeliminatedbypretreatmentofthedilutedantibodywith10mgofratNK1Rpeptideresidues393-407.
PhotoDescription:IHCimageofneuronsstainingfortheneurokinin1receptorintheratstriatum.Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:5000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host:Rabbit
Quantity/Volume:100µL
State:LyophilizedWholeSerum
ReactsWith:Rat
AvailABIlity:InStock
AlternateNames: Tachykininreceptor1;NK-1receptor;SPR,Substance-Preceptor;tachykininpeptidehormone;Tacr1r,anti-NK-1
GeneSymbol/ID,Accession#: Tacr1,24807
RRID: AB_10013880
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn