企业QQ
官方微信
TheImmunoStarneuronalnitricoxidesynthaseC-terminalantiserumwasqualitycontroltestedusingstandardimmunohistochemicalmethods.Theantiserumdemonstratesstronglypositivelabelingofrathypothalamus,striatum,cortexandspinalcordusingindirectimmunofluorescentandbiotin/avidin-HRPtechniques.Recommendedprimarydilutionis1/8,000-1/12,000inPBS/0.3%TritonX-100-Bn/Av-HRPTechnique.
ByWesternblotanalysisofbrainhomogenatestheantibodyspecificallylabelsabandofapproximately155kD.Immuno-labelingiscompletelyabolishedbypre-adsorptionwithsynthetichumannNOS(1419-1433)at5µgpermLofdilutedantibody.NocrossreactivitywithotherformsofNOSwereobserved.
PhotoDescription:IHCimageofasingleneuronstainingfortheC-terminalofnNOSintheratcortex(above),alongwithlowmagnificationofcortex(belowleft),neuronsintheratdentategyrus(belowmiddle)andinthestriatum(belowright).Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:10000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen,belowmiddleimagewithnickelstain.
Host: Rabbit
Quantity/Volume: 100µL
State:LyophilizedWholeSerum
ReactsWith:Cat,Chick,Ferret,GuineaPig,Hamster,Human,Monkey,Mouse,Opossum,Rat,Shrew,SquirrelMonkey,Vole,Zebrafish
AvailABIlity:InStock
AlternateNames: ConstitutiveNOS;NC-NOS;NOStypeI;NeuronalNOS;NitricOxideSynthase;bNOS;Nitricoxidesynthase-brain;N-NOS;Peptidyl-cysteineS-nitrosylase;IHPS1;NC-NOS;nitricoxidesynthase1(neuronal),anti-nNos:C-Terminal
GeneSymbol/ID,Accession#: NOS1,4842
RRID: AB_572256
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn