TheImmunoStarMuOpioidReceptorantiserumwasqualitycontroltestedusingstandardimmunohistochemicalmethods.Theantiserumdemonstratesstronglypositivelabelingofratcaudateputamenandspinalcord(dorsalhorn)usingindirectimmunofluorescentandbiotin/avidin-HRPtechniques.Recommendedprimarydilutionis1/6000-1/10000inPBS/0.3%TritonX-100-Biotin/avidin-HRPTechnique.
PreadsorptionwithMORpeptide(384-398)at10µg/mlcompletelyeliminateslabeling.Thespecificityoftheantiserumwasdeterminedbyimmunolabelingoftransfectedcells,WesternBlotanalysisandimmunoisolationstudies.
PhotoDescription: IHCimageofmuopioidreceptorstainingintheratstriatum.Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:10000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host: Rabbit
Quantity/Volume: 100µL
State:LyophilizedWholeSerum
ReactsWith: Cat,Chicken,Frog,GuineaPig,Human,Monkey,Mouse,Rat,Starling,Tadpole
AvailABIlity:InStock
AlternateNames: MOR-1;Mu-typeopioidreceptor;M-OR1;Muopiatereceptor;OpioidreceptorB;MUOR1;M-OR-1;MORA;oprm;opioidreceptor,mu1,anti-MOR
GeneSymbol/ID,Accession#: Oprm1,25601
RRID: AB_572251
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn