产品资料

Innova Biosciences/Lightning-Link® Alkaline Phosphatase (AP)/702-0005/1 reaction up to100ug

TheInnovaBiosciencesLightning-Link® AlkalinePhosphatase(AP) antibodylabelingkitenablesthedirectconjugationofantibodies,proteinsandpeptides,oranyotherbiomoleculewithanavailableaminegroup,to AlkalinePhosphatase(AP) withonly30secondshands-ontimeandwith100%recoveryofmaterials.Thetechnologyisfullyscalablefrom10ugto1gormoreandstringentlyQCtestedforconsistenthighqualityandexcellentbatch-to-batchreproducibility.

Lightning-Link_process_diagram

Alkalinephosphatase(AP)isahydrolaseenzymethatisfrequentlyconjugatedtoantibodiesforuseinimmunoassays.PNPPisapopularcolorimetricsubstrateforAP;theproductofthereaction,p-nitrophenol,canbereadat405nM.Fluorogenicsubstrates(e.g.4-methylumbelliferylphosphate;MUP)mayalsobeemployed.

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Immunohistochemistry(IHC)data

Lightning-Link® Alkaline Phosphatase (AP) IHC data

Figure1.DualstainingofCD3andCD20withantibodiesdirectlyconjugatedusingLightning-Linkkits.MouseantihumanCD3wasconjugatedwithAlkalinePhosphatase,andmouseanti-humanCD20wasconjugatedusingHRP.Bothantibodieswereusedsimultaneouslytostainformalin-fixed,paraffinembeddedhumanlymphnode.CD20positivecellsarevisualisedsuingDAB(Brown)andCD3cellswithFastRed(Red).Directlabelingofmonoclonalantibodiesenablesstraightforwarddual-colourstaininginIHC,whichisotherwiseahighlycomplexandtime-consumingtechnique.

ELISAdata

Figure2.AlkalinePhosphataseanti-HumanIgG(Fc)ConjugatesAnalyzedbyELISA.Anti-humanIgGmonoclonalantibodywaspurchasedpre-conjugatedtoalkalinephosphatasefromacommercialsupplier.Thesameantibodywaspurchasedinun-conjugatedformandthenconjugatedtoalkalinephosphataseusingaLightning-Link®kit.TheLightning-Link®conjugatedemonstratesenhancedtitreandsensitivity.

ScalABIlityData

Lightning-Link® Alkaline Phosphatase scability data

Figure3.GoatantiRabbitlabeledwithLightning-Link®AlkalinePhosphataseat25ug,250ug,2.5mgand25mgsinglereactionvialstestedwithRabbitIgGinasandwichELISA.Detection:PNPP.

StabilityData

Figure4.GoatantiRabbitlabeledwith4differentbatchesofLightning-Link®AlkalinePhosphataseat100ugreactionvialstestedwithRabbitIgGinasandwichELISA.Detection:PNPP.

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Innova Biosciences/Lightning-Link® Alkaline Phosphatase (AP)/702-0005/1 reaction up to100ug

Immunohistochemistry(IHC)data

ELISAdata

ScalABIlityData

StabilityData

ALP-functionalizedMesoporousSilicaNanoparticlesforAntibodyLabelinginImmunosensors-(2017)

Pilotstudyintomilkhaptoglobinasanindicatorofudderhealthinheifersaftercalving- ResearchinVeterinaryScience(2017)

Dramaticelevationinurinaryaminoterminaltitinfragmentexcretionquantifiedbyimmunoassayinduchennemusculardystrophypatientsandindystrophindeficientrodents- NeuromuscularDisorders(2017)

AnofilmslotblotforthedetectionofdevelopingP.falciparumoocystsinmosquitoes- PLoSOne(2017)

Serum-InfliximabTroughLevelsin45ChildrenwithInflammatoryBowelDiseaseonMaintenanceTreatment- IntJMolSci.(2017)

C-TerminallyTruncatedFormsofTau,ButNotFull-LengthTauorItsC-TerminalFragments,AreReleasedfromNeuronsIndependentlyofCellDeath - TheJournalofNeuroscience(2015)

Themajoracutephaseproteinsofbovinemilkinacommercialdairyherd - BMCVeterinaryResearch(2015)

Abiolayerinterferometry-basedassayforrapidandhighly-sensitivedetectionofbiowarfareagents- Biolayerinterferometry(2016)

Microarrayimmunoassayforthedetectionofgrapevineandtreefruitviruses-JournalofVirologicalMethods(2009)

InVivoStudiesofMutantFibrillin-1Microfibrils-JBC(2010)

FluorescentLabelingofAntibodyFragmentsUsingSplitGFP-PLoSOne(2011)

Broad-SpectrumInhibitionofHIV-1byaMonoclonalAntibodyDirectedagainstagp120-InducedEpitopeofCD4-PLoSOne(2011)

LargemolecularsizeEDTA-resistantcomplexescontainingS100A12,ERAC,inserumduringinflammatoryconditions-ScandinavianJournalofClinical&LaboratoryInvestigation(2012)

MultiplexDetectionofPlantPathogensUsingaMicrosphereImmunoassayTechnology-PLoSOne(2013)

SpecificexpressionpatternsandcelldistributionofancientandmodernPAGinbovineplacentaduringpregnancy-Reproduction(2013)

Troughs-infliximabandantibodiestowardsinfliximabinacohortof79IBDpatientswithmaintenanceinfliximabtreatment-JournalofCrohn"sandColitis(2014)

Alkalinephosphataselabeledantibody-basedelectRochemicalbiosensorforsensitiveHE4tumorMarkerdetection-ElectrochemistryCommunications(2015)

Improvedmethodsforthepurificationandenrichmentofbananastreakvirusforantibodyproductionandproteindetection-EuropeanJournalofPlantPathology(2015)