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OurInnovaCoat®GOLD40nmgoldconjugationkitsenabletheeasyconjugationofantibodies,proteinsoranyotherbiomoleculewithanaminegroup,tohighqualitygoldnanoparticleswithonly30secondshands-ontimeandwith100%recoveryofmaterials.
The40nmlyophilizedgoldnanoparticleswithinthekithaveaproprietarysurfacecoatthatcovalentlybindtheantibodyorproteinforminghighstableconjugatesThetechnologyisfullyscalableandstringentlyQCtestedforconsistenthighqualityandexcellentbatch-to-batchreproducibility.
Pleaserefertoour customservices forbulkordersandbioconjugationservice,or getincontact withustoknowmoreaboutthisproduct.
Figure1.40nmIC-GOLDlyophilizedMini,Midi,Maxi,andMegavialswereconjugatedtoanIgG:eachpacksizeyielded50ul,500ul,5ml,50mlof20ODgoldconjugaterespectively.TheresultingconjugateswereanalyzedbysandwichLateralFlowAssay,measuringthesignalintensityatdifferentantigenconcentrations.%CVcalculatedwas<5%.
Figure2.Fivedifferentbatchesof40nmInnovaCoat®GOLDlyophilizedMiniwereconjugatedtoanIgG.TheresultingconjugateswereanalyzedbysandwichLateralFlowAssay,measuringthesignalintensityatvariousantigenconcentrations.%CVcalculatedwas<5%.
Figure3.AntibodyconjugationusingInnovaCoat®GOLDvstraditionalpassiveconjugationtechniqueswithuncoatedgoldnanoparticles,showingbothenhancedsignalintensityandimprovedspecificity.40nmgoldparticleswerelabeledwithanti-IgAantibody,andusedtomeasureIgAconcentrationinalateralflowinhibitionassay,withIgAboundtoalateralflowstrip.
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