AtthecoreofthisproductisInnovaCoat®GOLD,auniquecoatednanoparticlethathasanavidlyboundprotectivesurfacecoatwhichcanwithstandthemostextremeconditions(e.g.2.5MNaOHat70°Cfor~2hours).
TheGoatAnti-RabbitIgGantibody(GAR)isirreversIBLyattachedtothesurfacecoatviaacovalentbond.Thisirreversibleattachmentdoesn’tallowthedesorptionoftheantibody,whichisamajorproblemwithproductsinwhichtheantibodyisonlypassivelyadsorbedtothemetal.
TheInnovaCoat®GOLDconjugatedtoGARantibodyrecognizesRabbitIgGwhichisoftenusedasaprimaryantibodyinvariousresearchapplications.Forthisreasonsecondaryantibodyanti-rabbitIgGscanbeusefultoolsfortheanalysisoftargetproteins.
Figure1.Lateralflowassayofour40nmInnovaCoat®GOLDconjugatedtoGoatAnti-RabbitIgG.ThesignalintensityoftheT-linkeclearlychangesinproportiontotheantigenconcentration.
Figure2.QuantificationoftheT-linesignalofthelateralflowassayinfigure1.
Figure3.40nmInnovaCoat®GOLDconjugatedtoGoatAnti-Rabbitrunonasandwichlateralflowassayinthepresence/absenceofRabbitIgG.PresenceofanantigencanbevisuallyassessedbyasignalontheT-Lineonthelateralflowassay.TheC-lineconfirmsthatanassayhasrunproperlyandshouldalwaysbevisible.
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