产品资料

Innova Biosciences/PiColorLock™/303-0125/2500/6250 assays

PiColorLock™phosphatedetectionreagentchangescolourinthepresenceofinorganicphosphate(Pi).ThiscanbeexploitedtomeasureanyenzymethatgeneratesPiincludingATPases,GTPases,phosphatases,heat-shockproteinsandDNAunwindingproteins.

TheuniqueformulationofPiColorLock™affordsenhancedassaylinearity,dynamicrangeandcolourstABIlity;itisalsopossIBLewithPiColorLock™toworkwithunstablesubstrates(e.g.ATP,GTP)thatgivehighnon-enzymaticbackgroundsignalswithotheracidicdye-baseddetectionreagents.

Features&benefits:

Colorimetricassay–Simple,scalableandnon-rADIoactive
Compatiblewithalmostanyassaybuffer–Flexibilityoverawiderangeofexistingassays
Stablereagentformulation–Longshelflife
Uniqueaccelerator–Speedsupcolordevelopment
Uniquestabilizer–Suppressesnonenzymaticbackgroundswithacid-labilesubstrates(ATPorGTP)
PiColorLock™-Picomplexisverystable–Noprecipitation,resultscanbemeasuredoverseveralhours–idealforHTS
Widelinearrange–Noinhibitionofcolordevelopmentbyhighconcentrationsofprotein
TheuniquePiColorLockTMStabilizerpreventsbackgrounddriftwithtime

ThePiColorLock™reagentisoftenusedwithunstablesubstrates(e.g.ATP,GTP)thatgiverisetonon-enzymaticbackgrounddriftwithtime.Theuniquestabilizer,providedinthePiColorLock™phosphatedetectionsystem,blocksthisnon-enzymaticbreakdowngeneratingastablelowbackground.Thestablesignalcanthereforebereaduptoseveralhoursafterthereactionhasended.

Figure1.Competitorassaysarebesetwithseveralproblemsincludingreagentprecipitation.ThehighstabilityofPiColorLock™ensureshighstabilityofthecoloreddye-phosphatecomplexes(greencolor).

Figure2.ATPhasbeenincubatedinthreedetectionreagents.Asteadilyrisingbackgroundsignalisseenwithcompetitorreagents,whereasPiColorLock™givesbaselinereadings.

Figure3.PiColorLock™hasalsobeendesignedtohavealargelinearrange,thusreducingtheneedforsampledilution.Competitors’productsarelinearoveramuchnarrowerrangeofconcentrations.

DisassemblyoftheStaphylococcusaureushibernating100SribosomebyanevolutionarilyconservedGTPase-PNAS(2017)
Viewreference
ConstructionofStructuralMimeticsoftheThyrotropinReceptorIntracellularDomain- BiophysJ.(2016)
Viewreference
Developmentofaone-potassayforscreeningandidentificationofMurpathwayinhibitorsinMycobacteriumtuberculosis-Nature(2016)
Viewreference
SelectiveassemblyofNa,K-ATPase?2?2heterodimersintheheart:distinctfunctionalpropertiesandisoform-selectiveinhibitors-JBiolChem(2016)
Viewreference
ReducedDynamin-relatedProtein1ProtectsAgainstPhosphorylatedTau-inducedMitochondrialDysfunctionandSynapticDamageinAlzheimer"sDisease -OxfordJournals (2016)
Viewreference
StructureoftheDictyosteliumMyosin-IIHeavyChainKinaseA(MHCK-A)[alpha]-kinasedomainapoenzymerevealsanovelautoinhibitedconformation-ScientificRep.(2016)
Viewreference
IndependentmechanismstargetSMCHD1toH3K9me3-modifiedchromatinandtheinactiveXchromosome - MolecularandCellularBIOLOGy(2015)
Viewreference
CharacterizationoftheCatalyticandNucleotideBindingPropertiesoftheAlpha-kinasedomainofDictyosteliumMyosin-IIHeavyChainKinaseA. - TheJournalofBiologicalChemistry(2015)
Viewreference
Digoxinderivativeswithselectivityforthe?2?3isoformofNa,K-ATPasepotentlyreduceintraocularpressure - ProcNatlAcadSciUSA(2015)
Viewreference
ConcertedanddifferentialactionsoftwoenzymaticdomainsunderlieRad5contributionstoDNAdamagetolerance - NucleicAcidsResearch(2015)
Viewreference
Embryodefective14encodesaplastid-targetedcGTPaseessentialforembryogenesisinmaize -PlantJ.(2015)
Viewreference
InsightintoStructure-FunctionRelationshipsandInhibitionoftheFattyAcyl-AMPLigase(FadD32)OrthologsfromMycobacteria - J.Biol.Chem.(2016)
Viewreference
Structure-BasedMutagenesisofSulfolobusTurretedIcosahedralVirusB204RevealsEssentialResiduesintheVirion-AssociatedDNA-PackagingATPase - J.Virol.(2016)
Viewreference
BenzimidazolecovalentprobesandthegastricH+/K+-ATPaseasamodelsystemforproteinlabelinginacopper-freesetting - MolecularBioSystems(2016)
Viewreference
PurificationoftheHuman?2IsoformofNa,K-ATPaseExpressedinPichiapastoris.StabilizationbyLipidsandFXYD1-Biochemistry(2007)
Viewreference
StabilizationofNa+,K+-ATPasePurifiedfromPichiapastorisMembranesbySpecificInteractionswithLipids-Biochemistry(2007)
Viewreference
CharacterizationofHumanGTPBP3,aGTP-BindingProteinInvolvedinMitochondrialtRNAModification-MolCellBiol(2008)
Viewreference
CharacterizationofStreptococcusgordoniiSecA2asaParalogueofSecA-JBacteriol(2009)
Viewreference
StructuralandMutationalAnalysesofDeinococcusradioduransUvrA2ProvideInsightintoDNABindingandDamageRecognitionbyUvrAs-Structure(2009)
Viewreference
IdentificationofaSmallMoleculeInhibitoroftheHumanDNARepairEnzymePolynucleotideKinase/Phosphatase-CancerRes(2009)
Viewreference
FunctionalstudyonGTPhydrolysisbytheGTP-bindingproteinfromSulfolobussolfataricus,amemberoftheHflXfamily-JBiochem(2010)
Viewreference
OligomericassemblyandinteractionswithinthehumanRuvB-likeRuvBL1andRuvBL2complexes-Biochem.J.(2010)
Viewreference
Anti-tubercularscreeningofnaturalproductsfromColombianplants:3-methoxynordomesticine,aninhibitorofMurEligaseofMycobacteriumtuberculosis-JAntimicrobChemother(2010)
Viewreference
RolesofElectrostaticsandConformationinProtein-CrystalInteractions -PLoSOne(2010)
Viewreference
SensorcombinationandchemometricvariableselectionforonlinemonitoringofStreptomycescoelicolorfed-batchcultivations-AppliedMicrobiologyandBiotechnology(2010)
Viewreference
MechanismofActionofanImidopiperidineInhibitorofHumanPolynucleotideKinase/Phosphatase-JBC(2010)
Viewreference
ATP-dependentMurEligaseinMycobacteriumtuberculosis:Biochemicalandstructuralcharacterisation-Tuberculosis(2010)
Viewreference
SelectivityofDigitalisGlycosidesforIsoformsofHumanNa,K-ATPase-JBC(2010)
Viewreference
Substrateuptake,phosphorusrepression,andeffectofseedcultureonglycopeptideantibioticproduction:Processmodeldevelopmentandexperimentalvalidation-BiotechnologyandBioengineering(2010)
Viewreference
Characterisationofmultiplesubstrate-specific(d)ITP/(d)XTPaseandmodellingofdeaminatedpurinenucleotidemetabolism-MIMSEPrints(2011)
Viewreference
Stabilizationofthe?2isoformofNa,K-ATPasebymutationsinaphospholipidbindingpocket-JBC(2011)
Viewreference
StructuralandfunctionalinsightsintoDNA-endprocessingbythearchaealHerAhelicase–NurAnucleasecomplex-NuclAcidsRes(2011)
Viewreference
FXYDProteinsStabilizeNa,K-ATPase,AmplificationOfSpecificPhosphatidylserine-ProteinInteractions-JBC(2011)
Viewreference
SepsisimpairsalveolarepithelialfunctionbydownregulatingNa-K-ATPasepump-AJP-LungPhysiol(2011)
Viewreference
StructuralandfunctionalinsightsintoDNA-endprocessingbythearchaealHerAhelicase–NurAnucleasecomplex-NucleicAcidsResearch(2011)
Viewreference
SepsisimpairsalveolarepithelialfunctionbydownregulatingNa-K-ATPasepump- AJP-LungPhysiol(2011)
Viewreference
PPM1HIsap27PhosphataseImplicatedinTrastuzumabResistance-CancerDiscovery(2011)
Viewreference
SynthesisandAntibacterialEvaluationofaNewSeriesofN-Alkyl-2-alkynyl/(E)-alkenyl-4-(1H)-quinolones-Molecules(2012)
Viewreference
Theunusualmycobacterialchaperonins:evidenceforinvivooligomerizationandspecializationoffunction-MolecularMicrobiology(2012)
Viewreference
KineticModellingofGlmUReactions–PrioritizationofReactionforTherapeuticApplication-PLoSOne(2012)
Viewreference
MesenchymalStemCellTherapy-HumanaPress(2013)
Viewreference
StructuralandMechaNISTicCharacterizationofL-HistidinolPhosphatePhosphatasefromthePHPFamilyofProteins-Biochemistry(2013)
Viewreference
PolyphosphatesinhibitextracellularmatrixmineralizationinMC3T3-E1osteoblastcultures-Bone(2013)
Viewreference
ANewlyUncoveredGroupofDistantlyRelatedLysineMethyltransferasesPreferentiallyInteractwithMolecularChaperonestoRegulateTheirActivity-PLoSGenetics(2013)
Viewreference
Na+RegulationintheMalariaParasitePlasmodiumfalciparumInvolvestheCationATPasePfATP4andIsaTargetoftheSpiroindoloneAntimalarials-CellHost&Microbe(2013)
Viewreference
Acalcineurindockingmotif(LxVP)indynamin-relatedprotein1contributestomitochondrialfragmentationandischemicneuronalinjury-TheJournalofBiologicalChemistry(2013)
Viewreference
CharacterisationofATP-DependentMurLigasesInvolvedintheBiogenesisofCellWallPeptidoglycaninMycobacteriumtuberculosis-PLoSOne(2013)
Viewreference
ATP-dependenttransportofstatinsbyhumanandratMRP2/Mrp2-ToxicologyandAppliedPharmacology(2013)
Viewreference
StructuralandfunctionalcharacterizationofaphosphatasedomainwithinyeastgeneraltranscriptionfactorTFIIIC-TheJournalofBiologicalChemistry(2013)
Viewreference
Plastiddivisioncontrol:thePDVproteinsregulateDRP5Bdynaminactivity-PlantMolecularBiology(2013)
Viewreference
ExtracellularATPReleasedbyOsteoblastsIsAKeyLocalInhibitorofBoneMineralisation-PLoSOne(2013)
Viewreference
DirectregulationofmicrotubuledynamicsbyKIF17motorandtaildomains-JournalofBiologicalChemistry(2013)
Viewreference
Anovelcholesterol-producingPichiapastorisstrainisanidealhostforfunctionalexpressionofhumanNa,K-ATPase?3?1isoform-AppliedMicrobiologyandBiotechnology(2013)
Viewreference
ChrysophaentinsarecompetitiveinhibitorsofFtsZandinhibitZ-ringformationinlivebacteria-Bioorganic&MedicinalChemistry(2013)
Viewreference
RNAsilencingofgenesinvolvedinAlzheimer"sdiseaseenhancesmitochondrialfunctionandsynapticactivity-BiochimicaetBiophysicaActa(2013)
Viewreference
AbiosensoroflocalkinesinactivityrevealsrolesofPKCandEB1inKIF17activation-JournalofCellBiology(2013)
Viewreference
3,4-Methylenedioxy-?-nitrostyreneinhibitsNLRP3activationbyblockingassemblyoftheinflammasome-JournalofBiologicalChemistry(2013)
Viewreference
Bordetellapertussisadenylatecyclasetoxintranslocationacrossatetheredlipidbilayer-PNAS(2013)
Viewreference
MutationsintheChromodomain-likeInsertionofTranslationElongationFactor3CompromiseProteinSynthesisThroughReducedATPaseActivity-JournalofBiologicalChemistry(2013)
Viewreference
ProspectingforUnannotatedEnzymes:Discoveryofa3?,5?-NucleotideBisphosphatePhosphatasewithintheAmidohydrolaseSuperfamily-Biochemistry(2013)
Viewreference
AblationofOsteopontinImprovestheSkeletalPhenotypeofPhospho1-/-Mice-JournalofBoneandMineralResearch(2014)
Viewreference
LowMolecularWeightProteinTyrosinePhosphatasesControlAntibioticProductioninStreptomycescoelicolorA3(2)-EnzymeEngineering(2014)
Viewreference
ThePIKEHomologCentauringammaRegulatesDevelopmentalTiminginDrosophila-PLoSOne(2014)
Viewreference
Quantificationofbiliaryexcretionandsinusoidalexcretionof5(6)-carboxy-2?,7?-dichlorofluorescein(CDF)inculturedhepatocytesisolatedfromSpragueDawley,WistarandMrp2-deficientWistar(TR?)rats-ToxicologyinVitro(2014)
Viewreference
Self-AssemblyofMinEontheMembraneUnderliesFormationoftheMinE-RingtoSustainFunctionoftheE.coliMinSystem-JournalofBiologicalChemistry(2014)
Viewreference
AllostericActivationofBordetellapertussisAdenylylCyclasebyCalmodulin:MolecularDynamicsandMutagenesisStudies-JournalofBiologicalChemistry(2014)
Viewreference
OptimizationofPyrrolamidesasMycobacterialGyrBATPaseInhibitors:Structure-ActivityRelationshipandInVivoEfficacyinaMouseModelofTuberculosis-AntimicrobialAgentsandChemotherapy(2014)
Viewreference
Assays,Surrogates,andAlternativeTechnologiesforaTBLeadIdentificationProgramTargetingDNAGyraseATPase-JournalofBiomolecularScreening(2014)
Viewreference
Acontinuousenzyme-coupledassayfortriphosphohydrolaseactivityofHIV-1restrictionfactorSAMHD1-AntimicrobialAgentsandChemotherapy(2014)
Viewreference
PreparationandcharacterizationofhumanADCK3,aputativeatypicalkinase-ProteinExpressionandPurification(2014)
Viewreference
FunctionalanalysisofTPMdomaincontainingRv2345ofMycobacteriumtuberculosisidentifiesitsphosphataseactivity-ProteinExpressionandPurification(2015)
Viewreference
Developmentofamitochondria-targetedHsp90inhibitorbasedonthecrystalstructuresofhumanTRAP1-JournaloftheAmericanChemicalSociety(2015)
Viewreference
Protein-pyridinolthioesterprecursorforbiosynthesisoftheorganometallicacyl-ironligandin[Fe]-hydrogenasecofactor-NatureCommunications(2015)
Viewreference
CrystalstructureoftheBloom"ssyndromehelicaseindicatesarolefortheHRDCdomaininconformationalchanges-NucleicAcidsResearch(2015)
Viewreference
ThemembraneremodelingproteinPex11pactivatestheGTPaseDnm1pduringperoxisomalfission-PNAS(2015)
Viewreference

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

Innova Biosciences/PiColorLock™/303-0125/2500/6250 assays

Features&benefits:

DisassemblyoftheStaphylococcusaureushibernating100SribosomebyanevolutionarilyconservedGTPase-PNAS(2017)

ConstructionofStructuralMimeticsoftheThyrotropinReceptorIntracellularDomain- BiophysJ.(2016)

Developmentofaone-potassayforscreeningandidentificationofMurpathwayinhibitorsinMycobacteriumtuberculosis-Nature(2016)

SelectiveassemblyofNa,K-ATPase?2?2heterodimersintheheart:distinctfunctionalpropertiesandisoform-selectiveinhibitors-JBiolChem(2016)

ReducedDynamin-relatedProtein1ProtectsAgainstPhosphorylatedTau-inducedMitochondrialDysfunctionandSynapticDamageinAlzheimer"sDisease -OxfordJournals (2016)

StructureoftheDictyosteliumMyosin-IIHeavyChainKinaseA(MHCK-A)[alpha]-kinasedomainapoenzymerevealsanovelautoinhibitedconformation-ScientificRep.(2016)

IndependentmechanismstargetSMCHD1toH3K9me3-modifiedchromatinandtheinactiveXchromosome - MolecularandCellularBIOLOGy(2015)

CharacterizationoftheCatalyticandNucleotideBindingPropertiesoftheAlpha-kinasedomainofDictyosteliumMyosin-IIHeavyChainKinaseA. - TheJournalofBiologicalChemistry(2015)

Digoxinderivativeswithselectivityforthe?2?3isoformofNa,K-ATPasepotentlyreduceintraocularpressure - ProcNatlAcadSciUSA(2015)

ConcertedanddifferentialactionsoftwoenzymaticdomainsunderlieRad5contributionstoDNAdamagetolerance - NucleicAcidsResearch(2015)

Embryodefective14encodesaplastid-targetedcGTPaseessentialforembryogenesisinmaize -PlantJ.(2015)

InsightintoStructure-FunctionRelationshipsandInhibitionoftheFattyAcyl-AMPLigase(FadD32)OrthologsfromMycobacteria - J.Biol.Chem.(2016)

Structure-BasedMutagenesisofSulfolobusTurretedIcosahedralVirusB204RevealsEssentialResiduesintheVirion-AssociatedDNA-PackagingATPase - J.Virol.(2016)

BenzimidazolecovalentprobesandthegastricH+/K+-ATPaseasamodelsystemforproteinlabelinginacopper-freesetting - MolecularBioSystems(2016)

PurificationoftheHuman?2IsoformofNa,K-ATPaseExpressedinPichiapastoris.StabilizationbyLipidsandFXYD1-Biochemistry(2007)

StabilizationofNa+,K+-ATPasePurifiedfromPichiapastorisMembranesbySpecificInteractionswithLipids-Biochemistry(2007)

CharacterizationofHumanGTPBP3,aGTP-BindingProteinInvolvedinMitochondrialtRNAModification-MolCellBiol(2008)

CharacterizationofStreptococcusgordoniiSecA2asaParalogueofSecA-JBacteriol(2009)

StructuralandMutationalAnalysesofDeinococcusradioduransUvrA2ProvideInsightintoDNABindingandDamageRecognitionbyUvrAs-Structure(2009)

IdentificationofaSmallMoleculeInhibitoroftheHumanDNARepairEnzymePolynucleotideKinase/Phosphatase-CancerRes(2009)

FunctionalstudyonGTPhydrolysisbytheGTP-bindingproteinfromSulfolobussolfataricus,amemberoftheHflXfamily-JBiochem(2010)

OligomericassemblyandinteractionswithinthehumanRuvB-likeRuvBL1andRuvBL2complexes-Biochem.J.(2010)

Anti-tubercularscreeningofnaturalproductsfromColombianplants:3-methoxynordomesticine,aninhibitorofMurEligaseofMycobacteriumtuberculosis-JAntimicrobChemother(2010)

RolesofElectrostaticsandConformationinProtein-CrystalInteractions -PLoSOne(2010)

SensorcombinationandchemometricvariableselectionforonlinemonitoringofStreptomycescoelicolorfed-batchcultivations-AppliedMicrobiologyandBiotechnology(2010)

MechanismofActionofanImidopiperidineInhibitorofHumanPolynucleotideKinase/Phosphatase-JBC(2010)

ATP-dependentMurEligaseinMycobacteriumtuberculosis:Biochemicalandstructuralcharacterisation-Tuberculosis(2010)

SelectivityofDigitalisGlycosidesforIsoformsofHumanNa,K-ATPase-JBC(2010)

Substrateuptake,phosphorusrepression,andeffectofseedcultureonglycopeptideantibioticproduction:Processmodeldevelopmentandexperimentalvalidation-BiotechnologyandBioengineering(2010)

Characterisationofmultiplesubstrate-specific(d)ITP/(d)XTPaseandmodellingofdeaminatedpurinenucleotidemetabolism-MIMSEPrints(2011)

Stabilizationofthe?2isoformofNa,K-ATPasebymutationsinaphospholipidbindingpocket-JBC(2011)

StructuralandfunctionalinsightsintoDNA-endprocessingbythearchaealHerAhelicase–NurAnucleasecomplex-NuclAcidsRes(2011)

FXYDProteinsStabilizeNa,K-ATPase,AmplificationOfSpecificPhosphatidylserine-ProteinInteractions-JBC(2011)

SepsisimpairsalveolarepithelialfunctionbydownregulatingNa-K-ATPasepump-AJP-LungPhysiol(2011)

StructuralandfunctionalinsightsintoDNA-endprocessingbythearchaealHerAhelicase–NurAnucleasecomplex-NucleicAcidsResearch(2011)

SepsisimpairsalveolarepithelialfunctionbydownregulatingNa-K-ATPasepump- AJP-LungPhysiol(2011)

PPM1HIsap27PhosphataseImplicatedinTrastuzumabResistance-CancerDiscovery(2011)

SynthesisandAntibacterialEvaluationofaNewSeriesofN-Alkyl-2-alkynyl/(E)-alkenyl-4-(1H)-quinolones-Molecules(2012)

Theunusualmycobacterialchaperonins:evidenceforinvivooligomerizationandspecializationoffunction-MolecularMicrobiology(2012)

KineticModellingofGlmUReactions–PrioritizationofReactionforTherapeuticApplication-PLoSOne(2012)

MesenchymalStemCellTherapy-HumanaPress(2013)

StructuralandMechaNISTicCharacterizationofL-HistidinolPhosphatePhosphatasefromthePHPFamilyofProteins-Biochemistry(2013)

PolyphosphatesinhibitextracellularmatrixmineralizationinMC3T3-E1osteoblastcultures-Bone(2013)

ANewlyUncoveredGroupofDistantlyRelatedLysineMethyltransferasesPreferentiallyInteractwithMolecularChaperonestoRegulateTheirActivity-PLoSGenetics(2013)

Na+RegulationintheMalariaParasitePlasmodiumfalciparumInvolvestheCationATPasePfATP4andIsaTargetoftheSpiroindoloneAntimalarials-CellHost&Microbe(2013)

Acalcineurindockingmotif(LxVP)indynamin-relatedprotein1contributestomitochondrialfragmentationandischemicneuronalinjury-TheJournalofBiologicalChemistry(2013)

CharacterisationofATP-DependentMurLigasesInvolvedintheBiogenesisofCellWallPeptidoglycaninMycobacteriumtuberculosis-PLoSOne(2013)

ATP-dependenttransportofstatinsbyhumanandratMRP2/Mrp2-ToxicologyandAppliedPharmacology(2013)

StructuralandfunctionalcharacterizationofaphosphatasedomainwithinyeastgeneraltranscriptionfactorTFIIIC-TheJournalofBiologicalChemistry(2013)

Plastiddivisioncontrol:thePDVproteinsregulateDRP5Bdynaminactivity-PlantMolecularBiology(2013)

ExtracellularATPReleasedbyOsteoblastsIsAKeyLocalInhibitorofBoneMineralisation-PLoSOne(2013)

DirectregulationofmicrotubuledynamicsbyKIF17motorandtaildomains-JournalofBiologicalChemistry(2013)

Anovelcholesterol-producingPichiapastorisstrainisanidealhostforfunctionalexpressionofhumanNa,K-ATPase?3?1isoform-AppliedMicrobiologyandBiotechnology(2013)

ChrysophaentinsarecompetitiveinhibitorsofFtsZandinhibitZ-ringformationinlivebacteria-Bioorganic&MedicinalChemistry(2013)

RNAsilencingofgenesinvolvedinAlzheimer"sdiseaseenhancesmitochondrialfunctionandsynapticactivity-BiochimicaetBiophysicaActa(2013)

AbiosensoroflocalkinesinactivityrevealsrolesofPKCandEB1inKIF17activation-JournalofCellBiology(2013)

3,4-Methylenedioxy-?-nitrostyreneinhibitsNLRP3activationbyblockingassemblyoftheinflammasome-JournalofBiologicalChemistry(2013)

Bordetellapertussisadenylatecyclasetoxintranslocationacrossatetheredlipidbilayer-PNAS(2013)

MutationsintheChromodomain-likeInsertionofTranslationElongationFactor3CompromiseProteinSynthesisThroughReducedATPaseActivity-JournalofBiologicalChemistry(2013)

ProspectingforUnannotatedEnzymes:Discoveryofa3?,5?-NucleotideBisphosphatePhosphatasewithintheAmidohydrolaseSuperfamily-Biochemistry(2013)

AblationofOsteopontinImprovestheSkeletalPhenotypeofPhospho1-/-Mice-JournalofBoneandMineralResearch(2014)

LowMolecularWeightProteinTyrosinePhosphatasesControlAntibioticProductioninStreptomycescoelicolorA3(2)-EnzymeEngineering(2014)

ThePIKEHomologCentauringammaRegulatesDevelopmentalTiminginDrosophila-PLoSOne(2014)

Quantificationofbiliaryexcretionandsinusoidalexcretionof5(6)-carboxy-2?,7?-dichlorofluorescein(CDF)inculturedhepatocytesisolatedfromSpragueDawley,WistarandMrp2-deficientWistar(TR?)rats-ToxicologyinVitro(2014)

Self-AssemblyofMinEontheMembraneUnderliesFormationoftheMinE-RingtoSustainFunctionoftheE.coliMinSystem-JournalofBiologicalChemistry(2014)

AllostericActivationofBordetellapertussisAdenylylCyclasebyCalmodulin:MolecularDynamicsandMutagenesisStudies-JournalofBiologicalChemistry(2014)

OptimizationofPyrrolamidesasMycobacterialGyrBATPaseInhibitors:Structure-ActivityRelationshipandInVivoEfficacyinaMouseModelofTuberculosis-AntimicrobialAgentsandChemotherapy(2014)

Assays,Surrogates,andAlternativeTechnologiesforaTBLeadIdentificationProgramTargetingDNAGyraseATPase-JournalofBiomolecularScreening(2014)

Acontinuousenzyme-coupledassayfortriphosphohydrolaseactivityofHIV-1restrictionfactorSAMHD1-AntimicrobialAgentsandChemotherapy(2014)

PreparationandcharacterizationofhumanADCK3,aputativeatypicalkinase-ProteinExpressionandPurification(2014)

FunctionalanalysisofTPMdomaincontainingRv2345ofMycobacteriumtuberculosisidentifiesitsphosphataseactivity-ProteinExpressionandPurification(2015)

Developmentofamitochondria-targetedHsp90inhibitorbasedonthecrystalstructuresofhumanTRAP1-JournaloftheAmericanChemicalSociety(2015)

Protein-pyridinolthioesterprecursorforbiosynthesisoftheorganometallicacyl-ironligandin[Fe]-hydrogenasecofactor-NatureCommunications(2015)

CrystalstructureoftheBloom"ssyndromehelicaseindicatesarolefortheHRDCdomaininconformationalchanges-NucleicAcidsResearch(2015)

ThemembraneremodelingproteinPex11pactivatestheGTPaseDnm1pduringperoxisomalfission-PNAS(2015)