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“We have used several LifeSensors products to investigate the role of the ubiquitin pathway in human cancer cells. I have been extremely impressed with the quality and reproducibility of their products. Their technical department are knowledgeable and their prompt assistance always enabled us to troubleshoot practical issues for diverse set of experiments.”
Khosrow Rezvani, MD, PhD, Associate Professor of Cancer Biology, USD
Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitylated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs protect polyubiquitinated proteins from deubiquitination and proteasomal degradation, allowing for detection at relatively low abundance. These properties effectively allow TUBEs to “capture” protein in its polyubiqutinated state. TUBEs can be used to isolate, enrich, and identify ubiquitinated proteins from cell and tissue extracts.
TUBE2 is based on UBAs from the protein ubiquilin. Magnetic beads coupled TUBE2 allow for efficient recovery of polyubiquitinated proteins in a single step without centrifuge. The magnetic TUBEs allows more complete removal of the unbound supernatant, which leads to lower background. Polyubiquitinated protein can easily be separated from TUBEs for proteomic studies or identification by Western blotting.
| Size | 1 ml |
| Buffer | PBS, pH 7.2, 20% ethanol |
| Recommended concentration | 10-20 µL of resin in 500 µL lysate (see manual) |
| Storage | Please keep the magnetic TUBE2 at 4°C. Do not centrifuge, dry or freeze the beads. |
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn