15N,heavy-labeledubiquitinprovidesastablestandardforubiquitinstructuralstudybyNMR.TherecombinanthumanubiquitinispurifiedfromE.coliexpressingthehumanubiquitingene.Thecellsaregrowninmediawherethesolenitrogensourceis15Nlabeledammoniumsulfate.Therecombinanthumanubiquitinispurifiedwithoutheatingbyaproprietaryprocedurethatpreservesthenativestructureoftheprotein.ThepurifiedproteinishomogenouswhenanalyzedbyoverloadedSDSgels.Thepoolsofpurifiedproteinsolutionareextensivelydialyzedagainstwaterandlyophilized.
Purity | 98%byRP-HPLC |
Protein | Ubiquitin |
Atom%Enrichment | 15N(98%) |
Molecularweight | 8,668.25Da |
Physicalstate | Lyophilizedpowder |
Quantity | 5mg,10mg |
Solubility | >30mg/mLinaqueoussolution |
Storage | -20°C |
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