Insulin-likeGrowthFactorstypeIandIIaresmallproteinsstructurallyrelatedtoinsulinthathavebothgrowthpromotinganddifferentiationactivityonavarietyofcells(1).TheiractionismediatedthroughbindingoftheIGF-Ireceptor(IGF-IR).IGF-IRisatetramerconsistingoftwoextracellular125kDaαchainsand2transmembrane95kDaβchains(2).IGF-IRhastyrosinekinaseactivtity,whichislinkedtotheras-raf-MAPKsignalingpathway.IGF-IandIGF-IIbindIGF-IRwithaKsofapproximately1-10nM(3).TwootherreceptorshavealsobeenreportedtobindIGFs:insulinreceptorandIGF-IIR.AnumberofIGFbindingproteinspresentinplasmaareinvolvedinIGFtransportandtheyappeartoalsoregulatetheinteractionofIGFswiththeirreceptorsbycontrollingtheamountoffreeIGFsavailableforreceptorinteractions(4).IGF-IRshavebeenimplicatedinpromotingtumorgrowththroughtheiranti-apoptoticaction.FurThermore,IGF-IRmayassociatewithcollagen-bindingintegrinstoregulatecelladhesion(5).ThesepropertiesmakethemonitoringofIGF-IRoncellsurfacesanimportantexerciseinunderstandingmalignantcelltransformationandtumorprogression.
MAB391isamonoclonaldirectedtotheextracellulardomainoftheIGF-IR.ThismonoclonalhasalsobeenshowntoneutralizeIGFactivityonthehumanMCFbreastepithelialtumorcellline.
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SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn