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Oxford Genetics/pSF-T7-EMCV (OG185) T7 IRES Expression Plasmid/OG185/1 Ea

PlasmidInfo:

PlasmidInformation

ProductName:pSF-T7-EMCV

ProductCode:OG185

Size(bp):4378bp

BacterialAntibioticSelection:KanR

OriginandCompatibility:pUChighcopyderivedfrompBR322

BacterialCopyNumber:500-700percell

Promoter:T7PolymerasePromoter

PlasmidPurpose:

Thisvectorallowstranscriptionofun-polyadenylatedandun-cappedRNAusingtheT7bacteriophagepolymerase.Thisplasmidalsocontainstheinternalribosomeentrysite(IRES)fromEncephalomyocarditisvirus(ECMV)betweenthepromoterandthemultiplecloningsite(MCS).ItisdesignedtobeusedtoexpressgenesusingeukaryoticT7expressionsystemssuchasthoseusedfortherecoveryofavarietyofviruses.ThisvectorisnotdesignedfortheexpressionoftwoproteinsfromthesamemRNA.ForthispurposewehavedesignedpSF-CMV-MCS-IRES-PciIwherethePciIsitecontainsastartcodoninthecorrectpositionfortheIREStoexpressefficiently.InthisvectorthereisanNcoIsite(whichcontainsanATGstartcodon)inthecorrectpositiontoenableefficientexpressionofageneintheMCS.

SequenceandMap:

OtherInfo:

TranscriptionTermination:

Thisplasmidcontainsthreealternativetranscriptionterminatorsformammalianbacterialandbacteriophage(T7)expression.Thismeansthatonlythepromoterneedstobechangedtoaltertheexpressionsystemyouareusing.Wesellmultiplepromotersthatcanbeusedineachofthesesystems.Thepresenceofeachterminatordoesnotreduceexpressioninthealternativesystems.

IPStatus:

IntellectualPropertyStatus

ThisproductispartofourSnapFastplasmidrange,formoreinformationontheIntellectualpropertystatusofthisplasmidpleaseclickhere

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