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AntibodiesOnline/MTOR ELISA Kit (Mechanistic Target of Rapamycin (serine/threonine Kinase))/ABIN274188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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AntibodiesOnline/MTOR ELISA Kit (Mechanistic Target of Rapamycin (serine/threonine Kinase))/ABIN274
Antigen
MechanisticTargetofRapamycin(serine/threonineKinase)(FRAP1)
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistictargetofrapamycin(serine/threoninekinase)
  • MTOR
  • Mtor
  • mtor
Alternatives
HumanMTORELISAKit
Kitswithalternativereactivityto:
23Human
15Rat(Rattus)
12Mouse(Murine)
1Chicken
1Cow(Bovine)
1Dog(Canine)
1GuineaPig
1Monkey
1Pig(Porcine)
1Rabbit
1Sheep(Ovine)
MethodType
CellELISA
Application
ELISA
Options
Bulkdiscount
Supplier
SupplierProductNo.
PurposeCell-BasedHumanmTOR(S2448)PhosphorylationELISAKit.Suitableforadherentwholecelllines.
SampleTypeCellCultureCells
AnalyticalMethodSemi-Quantitative
DetectionMethodColorimetric
SpecificityTheantibodiesprovidedinthiskitrecognizeshumanandmousemTORphosphorylatedatSerine-2448andtotalmTORforcomparison.
Characteristics
  • Siteandsignalpathway-specific
  • Invitrodetectionofadherentcellculture
  • Nosamplelysisneeded
  • CompatiblewithastandardELISAplatereader
  • FasterresultsthanwithELISA
  • Adaptableforhigh-throughputscreeninganddrugdiscovery
Components
  • uncoated96-wellMicroplate
  • WashBufferA
  • WashBufferB
  • FixingSolution
  • QuenchingBuffer
    • BlockingBuffer
  • Anti-phosphoantibody
  • Anti-panantibody
  • HRP-ConjugatedSecondaryAntibody
  • TMBOne-StepSubstrate
  • StopSolution
    • Materialnotincluded
    • Distilledordeionizedwater
    • 100 mLand1litergraduatedcylinders
    • Tubestopreparesampledilutions
    • ProteaseandPhosphataseinhibitors
    • Precisionpipettestodeliver2 μLto1 mLvolumes
    • Adjustable1-25 mLpipettesforreagentpreparation
    • Benchtoprockerorshaker
    • Microplatereadercapableofmeasuringabsorbanceat450nm
    AlternativeNamemTOR(FRAP1ELISAKitAbstract)
    BackgroundMechanistictargetofrapamycin,mammaliantargetofrapamycin(mTOR),FK506-bindingprotein12-rapamycin-associatedprotein1(FRAP1)
    GeneID2475
    UniProtP42345
    ResearchAreaCardiovascular,Cardiogenesis,CellCycle,DNA/RNA
    PathwaysPI3K-AktSignaling,RTKSignaling,AMPKSignaling,Interferon-gammaPathway,Fc-epsilonReceptorSignalingPathway,EGFRSignalingPathway,NeurotrophinSignalingPathway,RegulationofActinFilamentPolymerization,RegulationofMuscleCellDifferentiation,RegulationofCellSize,SkeletalMuscleFiberDevelopment,RegulationofCarbohydrateMetabolicProcess,Autophagy,CXCR4-mediatedSignalingEvents
    SampleVolume100μL
    PlateUncoated
    Protocol
    1. Seed10,000-30,000cellsintoeachwellandincubateovernight.
    2. Applyvarioustreatment,inhibitorsoractivatorsaccordingtomanufacturesinstructions.
    3. Add100 μLofFixingSolutionintoeachwellandincubatefor20 minatRTwithshaking.
    4. Add200 μLofprepared1XQuenchingBufferandincubate20 minatRT.
    5. Add200 μLofBlockingSolutionandincubatefor1hat37 °C.
    6. Add50 μLof1Xanti-phospho-proteinspecificantibodyoranti-pan-proteinspecificantibodytoeachwellandincubatefor2hatRT.
    7. Add50 μLofprepared1XHRP-Anti-RabbitorMouseIgGandincubatefor1hatRT.
    8. Add100 μLofTMBOne-StepSubstrateReagenttoeachwell.
    9. Incubate30 minatRT.
    10. Add50 μLofStopSolutiontoeachwell.
    11. Readat450nmimmediately.
    AssayProcedure

    Seed10,000-30,000cellsintoeachwellandincubateovernight.
    Applyvarioustreatment,inhibitorsoractivatorsaccordingtomanufacturesinstructions.
    Add100 μLofFixingSolutionintoeachwellandincubatefor20 minatRTwithshaking.
    Add200 μLofprepared1XQuenchingBufferandincubate20 minatRT.
    Add200 μLofBlockingSolutionandincubatefor1hat37 °C.
    Add50 μLof1Xanti-phospho-proteinspecificantibodyoranti-pan-proteinspecificantibodytoeachwellandincubatefor2hatRT.
    Add50 μLofprepared1XHRP-Anti-RabbitorMouseIgGandincubatefor1hatRT.
    Add100 μLofTMBOne-StepSubstrateReagenttoeachwell.
    Incubate30 minatRT.
    Add50 μLofStopSolutiontoeachwell.
    Readat450nmimmediately.

    RestrictionsForResearchUseonly
    Storage-20°C
    StorageCommentTheentirekitmaybestoredat-20°Cforupto6monthsfromthedateofshipment.Avoidrepeatedfreeze-thawcycles.
    ExpiryDate6months

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