Agrisera/PIP1;3 | Aquaporin, plasma membrane intrinistic protein 13/AS09 505/


productinformation
Background		PIP1s-isaplasmamembraneaquaporinewhichfacilitatestrasportofwateracrosscellmembrane.
Immunogen		KLH-conjugatedpeptidederivedfromOryzasativaPIP1s
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format
Quantity		100µl
Reconstitution
Storage		Storeat-20°C;makealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfrommaterialadheringtothecaporsidesofthetubes.
Testedapplications		ELISA(ELISA),WesternBlot(WB)
Relatedproducts		collectionofantibodiestoplasmamembraneproteins AS09504\|anti-PIP1;3\|aquaporin,plasmamembraneintrinisticprotein1-3 AS09506\|anti-PIP2;1\|aquaporin,plasmamembraneintrinisticprotein2-1 AS09507\|anti-PIP2;1\|aquaporin,plasmamembraneintrinisticprotein2-1 AS09508\|anti-PIP2;5\|aquaporin,plasmamembraneintrinisticprotein2-5 Secondaryantibodies
Additionalinformation		0.1%sodiumazideisaddedaspreservative.Forantibodyre-suspendinginformationcheckthetubelable. Antibodieswilldetecttargetproteininafew µgofacrudepreparationloadedperwell.Ifpurifiedpreparationsofvacuolarandplasmamembranesareused,oneµgloadperwellshouldbesufficient. Protocolforisolationofplasmamembraneisavailablehere.

applicationinformation
Recommendeddilution		1:8000(ELISA), 1:2000(WB)
Expected\|apparentMW		26-29kDa(Oryzasativa)
Confirmedreactivity		Oryzasativa
Predictedreactivity
Notreactivein		Hordeumvulgare
Additionalinformation		Proteinormembranesampleshouldbetreatedat70°Cfor10minbeforeloadingonthegel. Dilutedantibodysolutioncanbeused2to3timeswithinonemonthifit contains0.1%sodiumazideaspreservativeandisstoredat-20ºCto-80ºC. ManufacturedbyOperonBiotechnologies.
Selectedreferences		Zhangetal.(2017).ControlofsecondarycellwallpatterninginvolvesxylandeacetylationbyaGDSLesterase.NatPlants.2017Mar3;3:17017.doi:10.1038/nplants.2017.17.(Oryzasativa,westernblot) Liuetal.(2013).BrittleCulm1,aCOBRA-LikeProtein,FunctionsinCelluloseAssemblythroughBindingCelluloseMicrofibrils.PLoSGenet9(8):e1003704.doi:10.1371/journal.pgen.1003704(Oryzasativa,westernblot) Sakuraietal.(2008).Tissueandcell-specificlocalizationofriceaquaporinsandtheirwatertransportactivities-PlantCellPhysiol.49:30-39.

applicationexample

1.5µgofcrudemembranefraction/lanefromOryzasativaL.cv.Akitakomachiwereseparatedon12%SDS-PAGEandblotted1htoPVDFmembrane(40min.at10VusingBioRadsemidrytransfer).Filterswereblocked1hwith5%low-fatmilkpowderinTBS-T(0.05%TritonX.100).Membraneswerewashed5timeswithTBS-T,eachtimeinafreshpolystyreneboxandprobedwithanti-PIP1santibodies(AS09505,1:2000,1h)andsecondaryanti-rabbit(1:2000,1h).AllstepswereperformedinRTwithagitation.

Peptideusedtoelictithisantibodyhasbeenaddedtoprimaryantibodyincubation(rightpanel,++)andthereforespecificsignalhasbeendepletedinthisneutralization/immunocompetitionassay.

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Agrisera/PIP1;3 | Aquaporin, plasma membrane intrinistic protein 13/AS09 505/