QED Bioscience/Recombinant Human Interleukin1 Receptor Antagonist (rHuIL1ra)/40101P1000/1 Ea

Formulation:Sterile-filteredwhitelyophilizedpowderwithnoadditives.Purifiedbyproprietarychromatographictechniques.

Interleukin-1RAisamemberoftheInterleukin-1(IL-1)cytokinefamily.ItinhibitstheactivitiesofofIL-1alphaandIL-1betaandmodulatesavarietyofIL-1-relatedimmuneandinflammatoryresponses.Apolymorphismofthisgeneisreportedtobeassociatedwithanincreasedriskofosteoporoticfracturesandgastriccancer.

RecombinanthumanIL-1RAproducedinE.coliisanon-glycosylatedN-terminalmethionylformofthenaturally-occurringpolypeptidecontaining153aminoacidswithamolecularweightof17kDa.

Source:Escherichiacoli

Purity:Greaterthan98%asdeterminedbySDS-PAGEandRP-HPLC.

Accessionnumber:P18510.1

Aminoacidsequence:ThesequenceofthefirstfiveN-terminalaminoacidsisMet-Arg-Pro-Ser-Gly.

ED50bydose-dependentinhibitionofIL-1stimulationofD10Scellsis0.5ng/mlwhichcorrespondstoaSpecificActivityof2,000,000IU/mg.

ReconstituteinsteriledistilledH2Otonotlessthan100ug/mlwhichcanbefurtherdilutedinotheraqueoussolutions.

STORAGEANDSTABILITY

Storedessicatedbelow-18oC.Afterreconstitution,storeat4oCfor2-7daysandbelow-18oCforfutureuse.Forlongtermstorageofthereconstitutedproduct,additionof0.1%humanserumalbuminorbovineserumalbuminisrecommended.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.