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Prospec/OPG Human/Osteoprotegerin Human Recombinant/50µg/CYT-177-50µg-50µg

Cataloguenumber

CYT-177

Synonyms

TNFRSF11B,OPG,OCIF,Osteoclastogenesisinhibitoryfactor,Osteoprotegerin,TR1,MGC29565.

Introduction

Osteoprotegerinactsasdecoyreceptorforranklandtherebyneutralizesitsfunctioninosteoclastogenesis.OPGinhibitstheactivationofosteoclastsandpromotesosteoclastapoptosisinvitro.Bonehomeostasisseemstodependonthelocalrankl/opgratio.Osteoprotegerinmayalsoplayaroleinpreventingarterialcalcification.Mayactasdecoyreceptorfortrailandprotectagainstapoptosis.Trailbindingblockstheinhibitionofosteoclastogenesis.

Description

RecombinantHumanOsteoprotegerinproducedinE.colicellsisasingle,non-glycosylated,polypeptidechaincontaining174aminoacidsandhavingamolecularmassof20kDa.TheOPGispurifiedbyproprietarychromatographictechniques.

Source

EscherichiaColi.

PhysicalAppearance

SterileFilteredWhitelyophilized(freeze-dried)powder.

Formulation

TheOPGwaslyophilizedfroma0.2µmfilteredconcentrated(0.5mg/ml)solutioninPBS,pH7.4.

Solubility

ItisrecommendedtoreconstitutethelyophilizedOsteoprotegerininsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.

StABIlity

LyophilizedOsteoprotegerinalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionOCIFshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.

Purity

Greaterthan95.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.

BIOLOGicalActivity

TheactivityisdeterminedbyitsabilitytoneutralizethestimulationofU937cellstreatedwith10ng/mlofsolubleRANKLcorrespondingtoaspecificactivityof100,000IU/mg.

Aminoacidsequence

METFPPKYLHYDEETSHQLLCDKCPPGTYLKQHCTAKWKTVCAPCPDHYYTDSWHTSDECLYCSPVCKELQYVKQECNRTHNRVCECKEGRYLEIEFCLKHRSCPPGFGVVQAGTPERNTVCKRCPDGFFSNETSSKAPCRKHTNCSVFGLLLTQKGNATHDNICSGNSESTQK.

SafetyDataSheet

SDS

Usage

Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
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