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Prospec/MTHFD2 Human/MTHFD2 Human Recombinant/1mg/ENZ-853-1mg-1mg

Cataloguenumber

ENZ-853

Synonyms

Bifunctionalmethylenetetrahydrofolatedehydrogenase/cyclohydrolase,mitochondrial,NAD-dependentmethylenetetrahydrofolatedehydrogenase,Methenyltetrahydrofolatecyclohydrolase,NMDMC,MTHFD2.

Introduction

MTHFD2playsaroleasahomodimerwhichrequiresmagnesiumandinorganicphosphate.MTHFD2hasapseudogeneonchromosome7andowns3differentenzymaticactivities.Eachoftheactivitiescatalyzes1of3sequentialreactionsintheinterconversionof1-carbonderivativesoftetrahydrofolate,whicharesubstratesformethionine,thymidylate,anddenovopurinesyntheses.

Description

MTHFD2HumanRecombinantproducedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining344aminoacids(30-350)andhavingamolecularmassof37.2kDa.MTHFD2isfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.

Source

EscherichiaColi.

PhysicalAppearance

SterileFilteredclearsolution.

Formulation

TheMTHFD2solution(1mg/ml)containsPhosphatebufferedsaline(pH7.4),20%glyceroland1mMDTT.

StABIlity

Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.

Purity

Greaterthan95.0%asdeterminedbySDS-PAGE.

Aminoacidsequence

MGSSHHHHHHSSGLVPRGSHMGSLAAVRNEAVVISGRKLAQQIKQEVRQEVEEWVASGNKRPHLSVILVGENPASHSYVLNKTRAAAVVGINSETIMKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERRICNAVSPDKDVDGFHVINVGRMCLDQYSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMIKEGAAVIDVGINRVHDPVTAKPKLVGDVDFEGVRQKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRLEEREVLKSKELGVATN.

SafetyDataSheet

SDS

Usage

Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
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