Cataloguenumber
PRO-2172
Synonyms
Cytohesin1InteractingProtein,CytohesinBinderAndRegulator,PSCDBP,PleckstrinHomology,Sec7AndCoiled-CoilDomains,BindingProtein,PleckstrinHomologySec7AndCoiled-CoilDomains-BindingProtein,Cytohesin-AssociatedScaffoldingProtein,CytohesinBindingProteinHE,Cytohesin-BindingProteinHE,CbpHE,CASP,CYBR,HEPleckstrinHomology,Sec7AndCoiled/CoilDomains,BindingProtein,Cytohesin-1InteractingProtein,Cytohesin-InteractingProtein,CYTHIP,B3-1,CYTIP.
Introduction
Cytohesin-interactingprotein,alsoknownasCYTIPcontains2leucinezipperdomainsaswellasaputativeC-terminalnucleartargetingsignal.However,CYTIPdoesnotcontainanyhydrophobicregions.Inaddition,CYTIPisexpressedweaklyinrestingNKandTcells.ItalsomodulatestheactivationofARFgenesbyCYTH1.
Description
CYTIPHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining384aminoacids(1-359a.a)andhavingamolecularmassof42.6kDa.
CYTIPisfusedtoa25aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
E.coli.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
CYTIPproteinsolution(1mg/ml)containing20mMTris-HCl(pH8.0)and10%glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan85%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPPGSHMGSEFMSLQRLLQHSSNGNLADFCAGPAYSSYSTLTGSLTMDDNRRIQMLADTVATLPRGRKQLALTRSSSLSDFSWSQRKLVTVEKQDNETFGFEIQSYRPQNQNACSSEMFTLICKIQEDSPAHCAGLQAGDVLANINGVSTEGFTYKQVVDLIRSSGNLLTIETLNGTMILKRTELEAKLQVLKQTLKQKWVEYRSLQLQEHRLLHGDAANCPSLENMDLDELSLFGPLPGPGPALVDRNRLSSESSCKSWLSSMTMDSEDGYQTCVSEDSSRGAFSRQTSTDDECFIPKEGDDFLRRSSSRRNRSISNTSSGSMSPLWEGNLSSMFGTLPRKSRKGSVRKQLLKFIPGLHRAVEEEESRF
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
