企业QQ
官方微信
Catalogno.:34045Antigen:Bases2535-2835ofmouseROR2Cloneno.:ROR22535-2835fusedin-frametoMBPandexpressedinE.coli.Size:100uginPBS,pH7.4.HostSpecies:Mouse
PurifiedbyProteinGaffinitychromatography.AntibodyClass:IgG1
Specificity:MouseandhumanROR2.Doesnotcross-reactwithROR1.BACKGROUNDReceptortyrosinekinases(RTKs)arecellsurfacereceptorsthatregulatenormalcellularprocessesthroughligand-controlledtyrosinekinaseactivity.ROR2isamembrane-boundRTKthatisactivatedbyWntsignalingduringnormalboneandcartilagedevelopment.Recently,ROR2hasbeenshowntohavepro-tumOrigeniceffectsinosteosarcoma,melanoma,andrenalcellcarcinomacelllines.ROR2levelshavealsobeendescribedinsoft-tissuesarcomas;specifically,invitrostudiesrevealedthatinvasiveABIlitiesofleiomyosarcoma(LMS)andgastrointestinalstromaltumor(GIST)areaffectedbyROR2expression,andsuppressionofROR2reducesinvivotumormassinaxenotransplantationmodelofLMS.
Immunoblotting:useat1-10ug/ml.Abandof~105kDaisdetected.
Immunohistochemistry:useat1-10ug/mlonparaformaldehyde-fixed,paraffin-embeddedsectionsfollowingantigenretrieval(microwave12minincitratebuffer,pH6.0).
Thisantibodyisstableforatleastone(1)yearat-20oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.
DetectionofROR2inLMSandGIST.Sampleswerescored2(strongstaining),1(weakstaining),or0(absenceofstaining).SeereferenceEdrisB.etal.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplication.
PRODUCTREFERENCESForinvitroinvestigationaluseonly.NotMikelsAetal.2009JBiolChem284:30167-30176.foruseindiagnosticortherapeuticWrightTMetal.2009Oncogene28:2513-2523.applications.
EdrisBetal.2012JPathol227:223-233
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn