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QED Bioscience/RPA32/56216/1 Ea


productdatasheet


RPA32MonoclonalAntibody


ORDERINGINFORMATION

CatalogNo.:56216

Size:100ugProteinG-purifiedantibodyinPBS,pH7.4


SPECIFICATIONSUMMARY

Antigen:N-terminally6x-Histaggedfusionproteincorrespondingtofull-lengthhumanRPA32expressedin

E.coli.

HostSpecies:MouseAntibodyClass:IgG2a/κStABIlizers:None

Preservatives:None.Availableonrequest.


SPECIFICITY

ThisantibodyrecognizeshumanandmouseRPA32.RPA(ReplicationProtein

A)isasingle-strandedDNAbindingprotein.HumanRPAisaheterotrimericproteincontainingsubunitsof70,32,and14kD.ThisproteincomplexishighlyconservedineukaryotesandisessentialinDNAreplication,homologousrecombination,andnucleotideexcisionrepair.RPA32isphosphorylatedduringtheS-phaseofthecellcycle,inresponsetoDNAdamage,andduringapoptosisofJurkatT-lymphocytes.

APPLICATIONS

Immunoblotting,immunoprecipitation:useat0.2-1ug/ml.

Positivecontrol:HeLa,Raji,and3T3cellsandrecombinantprotein.


DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.


STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-70oC.Avoidmultiplefreeze-thawcycles.


REFERENCES

Iftode,Cetal.1999.CritRevBiochemMolBiol34:141-180;Bochkarev,Aetal.1999.

EMBO18:4498-4504;Dong,Jetal.1999.

BiochemJ337:311-317;Sibenaller,ZAetal.1998.Biochemistry37:12496-12506;Stigger,Eetal.1998.JBiolChem273:9337-9343;Bochkareva,Eetal.1998.JBiolChem273:3932-3936.


Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.


QEDBioscience,Inc.10919TechnologyPlace,SuiteC

SanDiego,CA92127

TollFree800.929.2114

Phone858.675.2405

Fax858.592.1509

info@qedbio.com

Visitourwebsiteforadditionalproductinformationandtoorderonline.

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