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QED Bioscience/Ski/34021/1 Ea


productdatasheet


Anti-skiMonoclonalAntibody


ORDERINGINFORMATION

CatalogNo.:34021(cloneG8)Size:100ugProteinG-purifiedantibodyinPBS,pH7.4


BACKGROUND

Theskioncogenefamily(skiandsno)encodestranscriptionalregulatorsthatcanaffectoncogenictransformationandcellulardifferenti-ation.skiandsnofunctionasco-activatorsorco-repressors,modulatingtranscriptionbyinter-actingwithtranscriptionfactorcomplexes.skiinteractswithmembersoftheNuclearFactorI(NFI)family,theretinoicacidreceptor,andthenuclearhormoneco-regulatorsNCoRandNCoA62/skip.


PRODUCT-SPECIFICREFERENCES

Tarapore,Petal.(1997)NucleicAcidsRes25:3895-3903.

Colmenares,Cetal.(1991)MolecularandCellularBIOLOGy11:1167-70.


Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

SPECIFICATIONSUMMARY

Immunogen:Recombinantv-skiHostSpecies:MouseAntibodyClass:IgG1StABIlizers:None

Preservatives:None.Availableonrequest.


SPECIFICITY

Thisantibodyrecognizesskiproteinofhuman,mouse,rat,chicken,andXenopus.


APPLICATIONS

Immunoblotting:useat1-5ug/ml.Predictedmolecularweightofv-skiis55kDa;full-lengthskirangesfrom~95kDaupto~115kDaforhyperphosphorylatedski.

Immunocytochemistry:useat1-10ug/ml.skiisanuclearprotein.

Immunoprecipitation:useat1-5ug/ml.Positivecontrols:Recombinantcelllinesexpressingskiproteins.


DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.


STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-70oC.Avoidmultiplefreeze-thawcycles.



QEDBioscience,Inc.10919TechnologyPlace,SuiteC

SanDiego,CA92127


TollFree800.929.2114

Phone858.675.2405

Fax858.592.1509

info@qedbio.com


Visitourwebsiteforadditionalproductinformationandtoorderonline.

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