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QED Bioscience/Thymidylate synthase/34001/1 Ea


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Anti-ThymidylateSynthaseMonoclonalAntibodies


ORDERINGINFORMATION

CatalogNo.:34001(2D8.D11)and34002(3A7.A11).

Size:200ugProteinG-purifiedantibodyinPBS,pH7.4.


BACKGROUND

Thymidylatesynthase(TS)isanintracellularenzymecriticalfordenovosynthesisofDNA.Incancer,expressionofTSisoftenelevatedandbecomesfurtherelevatedasaresultoftreatmentwiththemostcommonlyusedchemotherapeutic,5-fluorouracil(5-FU).Resistanceorlackofresponseto5-FUisattributedtotheelevationofTSactivity.OverexpressionofTShasbeendemonstratedinmanytypesoftumorsincludingbreast,colon,gastricandmelanomaandisusuallypredictiveofshortenedsurvivaltime.


SPECIFICATIONSUMMARY

Antigen:Recombinanthumanthymidylatesynthaseproducedin

E.coli.

HostSpecies:MouseAntibodyClass:IgG1StABIlizers:None

Preservatives:None.Availableonrequest.

SPECIFICITY

TheseantibodiesspecificallyrecognizehumanTS(36kDa).Theydonotcross-reactwithratTS.


APPLICATIONS

Immunohistochemistry:useat5-10ug/mlonfrozensections(reactivityonparaffin-embeddedsectionshasnotbeendetermined).

Immunoblotting:useat5-10ug/ml.ELISA:useat2-5ug/ml(optimizedforTSonsolidphaseat5ug/ml).

Immunoprecipitation:use#34002onlyat5-10ug/ml.


DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.


STORAGEANDSTABILITY

Thisantibodyisstableforatleastone

(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.


Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.



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SanDiego,CA92127


TollFree800.929.2114

Phone858.675.2405

Fax858.592.1509

info@qedbio.com


Visitourwebsiteforadditionalproductinformationandtoorderonline.

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