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QED Bioscience/Fibronectin/42042/1 Ea


HumanFibronectinMonoclonalAntibody


ORDERINGINFORMATION

CatalogNo.:42042(cloneFN30-8)Format:400ugin10mMPBS,pH7.4,1%BSA,lyophilized.


BACKGROUND

Fibronectinisa440kDaglycoproteinoftheextracellularmatrixthatbindstointegrins,collagen,fibrin,andheparansulfateproteoglycans.Fibronectinplaysamajorroleincelladhesion,growth,migration,anddifferentiation,anditisimportantforprocessessuchaswoundhealingandembryonicdevelopment.Alteredfibronectinexpression,degradation,andorganizationhasbeenassociatedwithanumberofpathologies,includingcancerandfibrosis.


SPECIFICATIONSUMMARY

Antigen:HumanplasmafibronectinHostSpecies:MouseAntibodysubtype:IgG1

Accessionno.:P02751,NP_001293058

Specificity:Thisantibodyrecognizesanepitopeinthecell-bindingdomainofhumanfibronectinandcaninhibitcelladhesion.


APPLICATIONS

ELISA:1-10ug/mlwithimmobilizedantigen.

WesternBlot:1-10ug/ml,reducingornon-reducingconditions.

CellAdhesionInhibitionAssay:0.1-1.0ug/mlon10ug/mlfibronectin-coatedmicrowellsinhibitsadhesionoftrypsinizedNRKcells.

Immunohistochemistry:1-10ug/ml,paraffin-embeddedorfrozentissuesectionswithProteinaseKtreatmentforantigenretrieval.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.


DILUTIONINSTRUCTIONS

Dissolvelyophilizedantibodyin200ulofdistilledwater(finalconcentration2.0mg/ml).


STORAGEANDSTABILITY

Thestocksolution(2.0mg/ml)canbestoredinaliquotsat-20oCfor1yearorcanbestoredat4oCfor6monthsafteradding0.1%sodiumazide.Preparedilutionsofstocksolutionimmediatelybeforeuse.Donotstoredilutedstocksolution.Avoidrepeatedfreeze-thawcycles.


Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures

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