ORDERINGINFORMATION
CatalogNo.:56270(cloneLy1C6)
Format:100uginPBS,pH7.4,purifiedbyProteinGaffinitychromatography,0.09%sodiumazide,50%glycerol.
BACKGROUND
Lysosome-associatedmembraneproteins(LAMP1andLAMP2)aremajorconstituentsofthelysosomalmembrane.Thestructuresofthesetwoproteinsarecloselyrelatedwith37%sequencehomology.Botharetransmembraneglycoproteinslocalizedprimarilyinlysosomesandlateendosomes.CellsurfaceLAMP1andLAMP2promoteadhesionofhumanperipheralbloodmononuclearcells(PBMC)tovascularendotheliumwhichsuggeststhattheyareinvolvedinadhesionofPBMCatsitesofinflammation.
SPECIFICATIONSUMMARY
Antigen:Ratliverlysosomemembranes.
HostSpecies:Mouse
AntibodyClass:IgG1
Specificity:Thisantibodyrecognizeshuman,rat,andhamsterLAMP1.
Accessionno.:NP_036989.1GeneID:25328
APPLICATIONS
Immunoblotting:Useat1ug/ml.Abandof
~120kDaisdetected.
Immunocytochemistry:Useat5-10ug/ml.
LAMP1detectedinratlivermicrosomes.ImmunofluorescentdetectionofLAMP1in
transfectedHeLacells.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplication.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
REFERENCE
LewisVetal.1985.JCellBiol100:1839-1847.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn