ORDERINGINFORMATION
CatalogNo.:56272(cloneGL2A7,ref.GrangerBLetal.1990,JBiolChem265:12036-12403)Format:100uginPBS,pH7.4,purifiedbyProteinGaffinitychromatography,0.09%sodiumazide,50%glycerol.BACKGROUND
Lysosome-associatedmembraneproteins(LAMP1andLAMP2)aremajorconstituentsofthelysosomalmembrane.Thesetwoproteinshavecloselyrelatedstructureswith37%sequencehomology.Botharetransmembraneglycoproteinslocalizedprimarilyinlysosomesandlateendosomes.LAMP2hasalsobeendetectedattheplasmamembraneofcellsundergoingdifferentiationandactivationandincellsthatsecretelysosomalhydrolases.CellsurfaceLAMP1andLAMP2promoteadhesionofhumanperipheralbloodmononuclearcells(PBMC)tovascularendotheliumwhichsuggeststhattheLAMPproteinsareinvolvedinadhesionofPBMCtositesofinflammation.DefectsinLAMP2areassociatedwithDanondisease.
SPECIFICATIONSUMMARY
Antigen:Gluteraldehyde-fixedmouseliverlysosomesHostSpecies:RatAntibodyClass:IgG1Specificity:Thisantibodyrecognizeshuman,mouse,andrabbitLAMP2(~100-110kDa).Accessionno.:NP_001017959.1GeneID:16784APPLICATIONS
Immunoblotting:Useat1-2ug/ml.Abandof~100-110kDa(glycosylated)isdetected.
Immunofluorescence:Useat1-2ug/ml.ThisantibodylabelslysosomesandlateendosomesincellspermeABIlizedwithsaponin.
Lane1:MWladder,Lane2:20ugHEK293lysate,Lane3:10ug3T3NIHlysate
Thesearerecommendedconcentrations.
Distributionoflysosomesincirculatingendothelialcells(CEC)
Endusershoulddetermineoptimalconcentrationsfortheirapplication.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
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GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn