ORDERINGINFORMATION
CatalogNo.Size
188301mg
18830-01100ug
Format:1mg/mlinPhosphateBufferedSaline,pH7.2,0.09%sodiumazide.
BACKGROUND
Enterokinase,alsoknownasenteropeptidase,isaserineproteasethatcatalyzesthehydrolysisofpeptidebondsinproteinsbut,unlikeotherkinases,doesnotcatalyzetransferofphosphategroups.Enterokinasecleavesproteinsfollowingalysineatanenterokinasecleavagesite,orECS(Asp-Asp-Asp-Asp-Lys).Invivo,thiscleavageresultsintrypsin-dependentactivationofotherpancreaticzymogens,suchaschymotrypsinogen,proelastase,procarboxypeptidaseandprolipaseinthelumenofthegut.
SPECIFICATIONSUMMARY
Antigen:DDDDKconjugatedtoKLH.
HostSpecies:Goat
Specificity:Antibodywasaffinity-purifiedonthepeptide.
APPLICATIONS
Immunoblotting:useatadilutionof1:1,000-1:20,000.
Immunocytochemistry:useatadilutionof1:100-1:400.
ELISA:fordetectionuseatadilutionof1:1,000-1:30,000;forcoatinguseatadilutionof1:100-1:500.
Immunoprecipitation:use1-4ug/mglysate.
Thesearerecommendeddilutions.
Endusersshoulddetermineoptimaldilutions
fortheirapplications.DetectionofECS-taggedproteinin200,100,and50ngofE.colilysatecontainingtaggedfusionproteinwith#18830diluted1:25,000.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat2-8oC.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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