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QED Bioscience/ECS/18830-01/1 Ea


ECS(DDDDK)Affinity-PurifiedPolyclonalAntibody


ORDERINGINFORMATION

CatalogNo.Size

188301mg

18830-01100ug

Format:1mg/mlinPhosphateBufferedSaline,pH7.2,0.09%sodiumazide.

BACKGROUND

Enterokinase,alsoknownasenteropeptidase,isaserineproteasethatcatalyzesthehydrolysisofpeptidebondsinproteinsbut,unlikeotherkinases,doesnotcatalyzetransferofphosphategroups.Enterokinasecleavesproteinsfollowingalysineatanenterokinasecleavagesite,orECS(Asp-Asp-Asp-Asp-Lys).Invivo,thiscleavageresultsintrypsin-dependentactivationofotherpancreaticzymogens,suchaschymotrypsinogen,proelastase,procarboxypeptidaseandprolipaseinthelumenofthegut.

SPECIFICATIONSUMMARY

Antigen:DDDDKconjugatedtoKLH.

HostSpecies:Goat

Specificity:Antibodywasaffinity-purifiedonthepeptide.

APPLICATIONS

Immunoblotting:useatadilutionof1:1,000-1:20,000.

Immunocytochemistry:useatadilutionof1:100-1:400.

ELISA:fordetectionuseatadilutionof1:1,000-1:30,000;forcoatinguseatadilutionof1:100-1:500.

Immunoprecipitation:use1-4ug/mglysate.


Thesearerecommendeddilutions.

Endusersshoulddetermineoptimaldilutions

fortheirapplications.DetectionofECS-taggedproteinin200,100,and50ngofE.colilysatecontainingtaggedfusionproteinwith#18830diluted1:25,000.


DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.


Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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