ORDERINGINFORMATION
CatalogNo.Size
188361mg
18836-01100ug
Format:1mg/mlinPhosphateBufferedSaline(PBS),0.09%sodiumazide.
BACKGROUND
TheGlu-GlutagrepresentsthepeptidesequenceEYMPMEwhichisderivedfromthemiddleTantigenofpolyomavirus.Giventhesmallsizeofthispeptide,itisunlikelythatmodifyingproteinswithitwillaffectproteinstructureorfunction.
SPECIFICATIONSUMMARY
Antigen:Syntheticpeptidecorrespondingtoaa314-319(EYMPME)ofmiddleTantigenofmousepolyomavirusconjugatedtoKLH.
HostSpecies:Goat
Specificity:Antibodywasaffinity-purifiedonthepeptide.
APPLICATIONS
Immunoblotting:useatadilutionof1:1,000-1:20,000.
Immunocytochemistry:useatadilutionof1:100-1:400.
Immunoprecipitation:use1-4ug/mglysate.ELISA:fordetectionuseatadilutionof1:1,000-1:30,000;forcoatinguseatadilutionof1:100-1:500.
Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.
DetectionofGlu-Glu-taggedproteinin200,100,and50ngofE.colilysatecontainingtaggedfusionproteinwith#18836diluted1:5,000.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat2-8oC.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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