Active Motif/组蛋白和修饰组蛋白/31293

Active Motif offers a wide variety of recombinant histones that include site- and degree-specific modifications such as methylation, acetylation and phosphorylation. The combination of histone post-translational modifications form the basis of the “histone code” that serves to regulate a variety of nuclear functions, including interactions with chromatin-associated proteins, nucleosome remodeling, transcriptional regulation, replication and DNA repair. Each recombinant histone is prepared using one of two patented technologies: Expressed Protein Ligation (EPL) or Methylated Lysine Analog (MLA). We also offer a subset of our histone H3 proteins that have been biotinylated for use in FRET assays and other capture techniques.

With our MLA technology, methylated histones are generated via a chemical alkylation reaction that introduces a methyl-lysine analog at the desired lysine location, giving us precise control over the site and degree of methylation. Alternatively, the EPL technology can be used to generate methylated, acetylated and phosphorylated histones. Using EPL, the histone globular domain is ligated to a peptide that contains the N-terminal histone tail with the desired modifications. This ligation reaction maintains the native histone bonds. Both methods produce proteins that are validated to be over 98% pure. For more information on the EPL and MLA technologies, click on the EPL and MLA Technologies tab below.

Active Motif also offers a number of biotinylated recombinant histone H3 proteins. Biotin is linked either to unmodified or modified recombinant H3 protein at its N-terminus via a carbon linker. The addition of biotin enables the recombinant histones to be used as a substrate for capture of protein binding interactions. Biotinylated histone H3 proteins are also ideal substrates for homogenous FRET assays. Simply incubate the recombinant histone with the enzyme of interest and detect using streptavidin-coated donor beads and antibody-conjugated acceptor beads.

A complete list of recombinant histones is shown below. Click on the protein name to see complete information.