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Reaction Biology/PB1-1 (GST)/RD-11-234

DESCRIPTION:

Human recombinant PB1-1 bromodomain expressed as an N-terminal GST-fusion protein in E.coli. PB1, a chromatin targeting component of the PBAF chromatin-remodeling complex, comprises six tandem bromodomains. Mutations in PB1, including PB1-1 occur frequently in renal cancers. Among the acetylated lysines of histone N-terminal tails, PB1-1 binds preferentially to H3K14aC. Crystal structures of PB1-1, both in complex with H3K14Ac peptide and isolation have been determined.

ACCESSION #:NM_018313

Uniprot Link

INCLUDES AMINO ACIDS:23-156

TAG(S):N-terminal GST tag

MW: 43.0 kDa

EXPRESSION SYSTEM: E.coli

SUPPLIED AS:Solution of purified recombinant protein in 50 mM Tris HCl, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10 % glycerol

STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.

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Substantial Discounts Available on Bulk Quantities
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