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DESCRIPTION:Human recombinant PB1-5 bromodomain (fifth bromodomain of polybromo-1). PB1, a chromatin-targeting component of the PBAF (SWI/SNF-B) chromatin-remodeling complex, comprises six tandem bromodomains. Mutations in PB1, including in PB1-5, occur frequently in renal cancers. PB1-5 shows binding affinity for a singly acetylated histone H2B peptide (H2BK85Ac).
ACCESSION #: NM_018313
Uniprot Link
INCLUDES AMINO ACIDS: 645-766
TAG(S): N-terminal His-tag
MW: 17.1 kDa
EXPRESSION SYSTEM: E. coli
SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris HCl, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v)
STORAGE: -70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.
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