Type
Recombinant
Description
Matrixmetalloproteinases(MMPs)areafamilyofendoproteasesthatrequirezincandcalciumforexpressingcatalyticactivity.Theseenzymesplayacentralroleinthemaintenanceandremodelingoftheextracellularmatrix.Elevatedexpressionoftheiractivity,causedeitherbyup-regulationoftheirexpressionordown-regulationoftheircognateinhibitors,hasbeenimplicatedinvariousdegenerativedisorders,includingarthritis,cardiovasculardisease,skeletalgrowth-platedisorders,andcancermetastasis.MMP-1 isasecretedcollagenasewithspecificitytowardTypeI,II,III,VII,andXcollagens.RecombinantHumanMMP-1 isa42.7 kDaproteincontainingtheentirecatalyticN-terminaldomainandtheC-terminaldomain,whichisinvolvedinsubstratespecificity,andinbindingTIMP-1.
AminoAcidSequence
MFVLTEGNPRWEQTHLTYRIENYTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGDHRDNSPFDGPGGNLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAAHELGHSLGLSHSTDIGALMYPSYTFSGDVQLAQDDIDGIQAIYGRSQNPVQPIGPQTPKACDSKLTFDAITTIRGEVMFFKDRFYMRTNPFYPEVELNFISVFWPQLPNGLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGYPKDIYSSFGFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDFPGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCRKN
Source
E.coli
Purity
90%
BIOLOGicalActivity
MMP-1 activitywasmeasuredbyitsABIlitytocleaveachromogenicpeptideMMP-1 substrateatroomtemperature.AtanMMP-1 concentrationof2.5 µg/ml,50%cleavagewasachievedatanincubationtimeofapproximately25 minutes.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.5–1.0 mg/ml.Note:Slowtodissolve.Gentlyshakethesolutionforabout10 minutesforbettersolubility.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C