Type
Recombinant
Description
NAP-2 isaCXCchemokinethatcansignalthroughtheCXCR1andCXCR2receptors.Itisproducedinleukocytesbyenzymaticprocessingofaprecursorcalledplateletbasicprotein(PBP).NAP-2 chemoattractsandactivatesneutrophils.RecombinantHumanNAP-2 proteinisa7.6 kDaproteincontaining70 aminoacidresidues,includingthefourhighlyconservedcysteineresiduespresentinCXCchemokines,andalsothe“ELR”motif,commontoCXCchemokinesthatbindtoCXCR1and CXCR2.
AminoAcidSequence
AELRCMCIKTTSGIHPKNIQSLEVIGKGTHCNQVEVIATLKDGRKICLDPDAPRIKKIVQKKLAGDESAD
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytochemoattracthumanneutrophilsusingaconcentrationrangeof1.0–10.0 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C