Type
Recombinant
Description
NT-3 isaneurotrophicfactorstructurallyrelatedtoβ-NGF,BDNF,andNT-4. Theseproteinsbelongtothecysteine-knotfamilyofgrowthfactorsthatassumestabledimericstructures.NT-3 isexpressedbyneuronsofthecentralnervoussystems,andcansignalthroughthetrkreceptors.NT-3 promotesthegrowthandsurvivalofnerveandglialcells.Theaminoacidsequencesofhuman,murineandratNT-3 areidentical.RecombinantHumanNT-3 isanoncovalentlylinkedhomodimeroftwo13.6 kDapolypeptidemonomers(240 totalaminoacidresidues).
AminoAcidSequence
MYAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50asdeterminedbythedose-dependentinductionofcholineacetyltransferaseactivityinratbasalforebrainprimaryseptalcellcultureswasfoundtobeintherangeof20–50 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C