Type
Recombinant
Description
SecretedFrizzledRelatedProteins(sFRPs)modulateWNTsignalingbybindingdirectlytoWNTproteinsinamannerthataffectstheirreceptorbindingandsignalingcapABIlities.sFRP-1 isawidelydistributedproteinthatcanbinddirectlytoWNT1,WNT2,andpossIBLyotherWNTproteins,andgenerallyexertsanti-proliferativeeffectsconsistentwithactivityasaWNTantagoNIST.Italsoinhibitsapoptosis,andhasbeenfoundtobedown-regulatedinmanysolidtumors,butup-regulatedinuterineleiomyomas.RecombinantHumansFRP-1 isaglycosylated283 aminoacidproteincontainingacysteine-richFrizzledhomologousdomain.ThecalculatedmolecularweightofRecombinantHumansFRP-1 is32.5 kDa.
AminoAcidSequence
SEYDYVSFQSDIGPYQSGRFYTKPPQCVDIPADLRLCHNVGYKKMVLPNLLEHETMAEVKQQASSWVPLLNKNCHAGTQVFLCSLFAPVCLDRPIYPCRWLCEAVRDSCEPVMQFFGFYWPEMLKCDKFPEGDVCIAMTPPNATEASKPQGTTVCPPCDNELKSEAIIEHLCASEFALRMKIKEVKKENGDKKIVPKKKKPLKLGPIKKKDLKKLVLYLKNGADCPCHQLDNLSHHFLIMGRKVKSQYLLTAIHKWDKKNKEFKNFMKKMKNHECPTFQSVFK
Source
HeLacells
Purity
97%
BIOLOGicalActivity
DeterminedbyitsabilitytoinhibitBMP-2 proliferationeffectofATDC-5 cells. TheexpectedED50is0.3–0.5 µg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C