Type
Recombinant
Description
TumorNecrosisFactor-aRatRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining157 aminoacidsandhavingamolecularmassof17339.44 Dalton.TheTNF-alphaispurifiedbystandardchromatographictechniques.
AminoAcidSequence
ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeMet-Leu-Arg-Ser-Ser.
Source
E.coli
Purity
Greaterthan97.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
BIOLOGicalActivity
TheED50asdeterminedbythecytolysisofmurineL929cellsinthepresenceofActinomycinDis<0.05 ng/ml,correspondingtoaSpecificActivityof5×107 IU/mg.
Formulation
Theproteinwaslyophilizedwithnoadditives.
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
LyophilizedTumorNecrosisFactor-aalthoughstableatroomtemperaturefor3 weeks,shouldbestoreddesiccatedbelow –18°C.UponreconstitutionTNF-ashouldbestoredat4°Cbetween2–7daysandforfutureusebelow –18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
PhysicalAppearance
Sterilefilteredwhitelyophilized(freeze-dried)powder.