Product Description
Size: 100 μl
Ab Type: Monoclonal
Reactivity: Eukaryotes
Immunogen: Acetylated H2B (Lys16)-KLH
Host: Mouse
Product DescriptionThis product was produced by immunizing balb/c mice with a synthetic acetyl peptide corresponding to residues surrounding Lys16 of human histone H2B. Antibodies were purified by protein G-conjugated agarose followed by acetylated histone H2B (Lys16) peptide affinity chromatography (H2BK16ac).
SpecificityThis antibody detects histone H2B only when it is acetylated at Lys16, but not the the acetylated H2B peptide at Lys15, crotonylated peptide at Lys16 orthe unmodified peptide.
Recommended ApplicationsELISA, WB, ChIP, IPWB=Western blotting; ChIP=Chromatin immunoprecipitation; IP: Immunoprecipitation
Recommended DilutionWB:1:2000.
IMPORTANT NOTESFor Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 at room temperature two hours with gentle shaking.Optimal dilutions/concentrations should be determined by the end user and maybe assay dependent.
A. Dot blotting analysis on indicated amount of acetylated H2B peptide at Lys16 (Lane 1), acetylated H2B peptide at Lys15 (Lane2), crotonylated H2B peptide at Lys16 (Lane3), and unmodified H2B peptide at Lys16 (Lane 4) using acetyl-histone H2B (Lys16) mouse mAb. B. Western blotting analysis on 30 µg of crude proteins from HeLa whole cell lysates with (Lane2) or without (Lane1) treatment of sodium butyrate (30 mM, 4hours) using acetyl-histone H2B (Lys16) mouse mAb (1:2000).
Scientific DescriptionThe ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code”. Lysine acetylation in core histones occurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation, and nuclear import. In most species, histone H2A is primarily acetylated at Lys5, 9, 15 and 36; H2B is primarily acetylated at Lys5, 12, 15, 16 and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56 and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16 and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of histone lysine acetylation status is impaired in cancers and other diseases. Therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs.
Storage & StabilityThe antibody is delivered in lyophilized powder. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds, and then reconstitute it with the antibody stabilizer provided. The stabilizer contains PBS, 50% glycerol, and 0.01% sodium azide. Please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Antibody is stable for 12 months from date of reconstitution. Equilibrate the antibody to room temperature for 2 minutes and mix by gently pipetting before use.**Research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.