General Information | |||||
Catalog number | PTM-117 | ||||
Size | 100 μl at 5.0 mg/ml | ||||
Clonality | Polyclonal | ||||
Species reactivity | Human, Mouse, Rat | ||||
Host species | Rabbit | ||||
Immunogen | H3K36ac-KLH | ||||
Form | Liquid | ||||
Product Information | |||||
Application | ELISA, Dot Blot, Western Blot, IHC | ||||
Dilution | Western Blot | 1:2000 | |||
IHC | 1:200 | ||||
Images | |||||
Dot blot data | |||||
NO. | Peptide | NO. | Peptide | ||
Lane1 | H3K36ac | lane 2 | H3K36un | ||
Dot blotting analysis indicated the amount of peptides using histone H3K36ac rabbit pAb. The list of peptides is included in the above table. | |||||
WB data | |||||
Western blotting analysis on 30 μg of crude proteins from human HeLa whole cell lysates with (right) or without (left) treatment of sodium butyrate (30 mM, 4 hours) using acetyl-histone H3 (Lys36) rabbit pAb (1: 2000). | |||||
IHC data | |||||
Immunohistochemical analysis of paraffin-embedded human neuroblastoma tissue, labeling H3K36ac with PTM-117 at 1/200 dilution.Nuclear staining on tumor cells of human neuroblastoma was observed. Counter stained with hematoxylin. | |||||
Scientific Description | |||||
The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity.The amino-terminal tails of core histones undergo lysine acetylationin multiple sites, termed as “histone code”.Lysine acetylation in core histone soccurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation, and nuclear import.. In most species, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16,and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56,and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Morethan 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs)have been identified to date, while the mechanistic details of substrateselection and site specificity of these enzymes remain unclear. The regulation of histonelysine acetylation status is impaired in the pathologies of cancerand other diseases and therefore, enzymes regulating histone lysine acetylationhave become promising targets for anti-cancer drugs. | |||||
Source/Purification | |||||
This product is produced by immunizing rabbits with a synthetic acetyl peptide corresponding to residues surrounding Lys36 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by acetylated histone H3 (Lys36) peptide affinity chromatography. | |||||
Storage & Stability | |||||
The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage. | |||||
References | |||||
1. Zhang K, Chen Y, Zhang Z, et al. J Proteome Res. 2009, 8(2):900-9062. Cheng Z, Tang Y, Chen Y, et al. Mol Cell Proteomics. 2009, 8(1):45-52.3. Chen Y, Sprung R, Tang Y, et al. Mol Cell Proteomics. 2007, 6(5):812-819.**Research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals. |
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