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Ptmbiolabs/Acetyl-Histone H3 (Lys4) rabbit pAb/100 μl/PTM-124188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Ptmbiolabs/Acetyl-Histone H3 (Lys4) rabbit pAb/100 μl/PTM-124

Product DescriptionSize: 100 μlAb Type: PolyclonalReactivity: EukaryotesImmunogen: Acetylated H3 (Lys4)-KLHHost: Rabbit
Product DescriptionThis product was produced by immunizing rabbits with a synthetic acetylpeptide corresponding to residues surrounding Lys4 of human histone H3. Antibodies were purified by protein A-conjugated agarose followed by acetylated histone H3 (Lys4) peptide affinity chromatography.SpecificityThis antibody detects histone H3 only when it is acetylated at Lys4. This antibody has been shown to selectively recognize acetylated H3 peptide at Lys4,but not the structurally similar crotonylated peptide at Lys4, acetylated,butyrylated and crotonylated peptide at Lys9.Recommended ApplicationsELISA, WB, ChIP, IP,IHCWB=Western blotting; ChIP=Chromatin Immunoprecipitation; IP: Immunoprecipitation;IHC:ImmunohistochemistryRecommended dilutionWB:1:2000.IMPORTANT NOTESFor Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 at room temperature two hours with gentle shaking.
PTM-124A. Dot blotting analysis on indicated amount of acetylated H3 peptide at Lys4 (Lane 1), crotonylated H3 peptide at Lys4 (Lane 2), acetylated peptide at Lys9 (Lane 3), butyrylated peptide at Lys9 (Lane 4), crotonylated peptide at Lys9 (Lane 5),and unmodified H3 peptide at Lys4 (Lane 6) using acetyl-histone H3 (Lys4) rabbit pAb.B. Western blotting analysis on 30 µg of crude proteins from HeLa wholecell lysates with (Lane 2) or without (Lane 1) treatment of sodium butyrate (30mM, 4 hours) using acetyl-histone H3 (Lys4) rabbit pAb (1:2000).
Scientific DescriptionThe ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites. Lysine acetylation in core histones occurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, transcription regulation, cell cycle progression, apoptosis, differentiation, and nuclear import. In most species, histone H2A is primarily acetylated at Lys5, 9, 15 and 36; H2B is primarily acetylated at Lys5, 12, 15, 16 and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56 and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16 and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of histone lysine acetylation status is impaired in cancer and other patients and therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs.Storage & StabilityThe antibody is delivered in lyophilized powder. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds, and then reconstitute it with the antibody stabilizer provided. The stabilizer contains PBS, 50% glycerol, and 0.01% sodium azide. Please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Antibody is stable for 12 months from date of reconstitution. Equilibrate the antibody to room temperature for 2 minutes and mix by gently pipetting before use.

**Research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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