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Ptmbiolabs/Acetyl-Histone H4 (Lys8) rabbit pAb/PTM-120188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Ptmbiolabs/Acetyl-Histone H4 (Lys8) rabbit pAb/PTM-120

Product DescriptionSize: 100 μlAb Type: PolyclonalReactivity: EukaryotesImmunogen: Acetylated H4 (Lys8)-KLHHost: Rabbit
Product DescriptionThis product is produced by immunizing rabbits with a synthetic acetyl peptide corresponding to residues surrounding Lys8 of human histone H4. Antibodies are purified by protein A-conjugated agarose followed by acetylated histone H4 (Lys8) peptide affinity chromatography.SpecificityThis antibody only recognizes acetylated H4 peptide at Lys8, but not the acetylated H4 peptide at Lys5, structurally similar butyrylated or crotonylated peptide at Lys5 and Lys8.Recommended ApplicationsELISA, WB, ChIP, IPWB=Western blotting; ChIP=Chromatin Immunoprecipitation; IP: ImmunoprecipitationRecommended dilutionWB:1:2000.IMPORTANT NOTESFor Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 at room temperature two hours with gentle shaking.
PTM-120A. Dot blotting analysis on indicated amount of acetylated H4 peptide at Lys8 (Lane 1), acetylated H4 peptide at Lys5 (Lane 2), butyrylated H4 peptide at Lys5 (Lane 3), butyrylated H4 peptide at Lys8 (Lane 4), crotonylated peptide at Lys8 (Lane 5) and unmodified H4 peptide (Lane 6) using acetyl-histone H4 (Lys8) rabbit pAb.B. Western blotting analysis on 30 µg of crude proteins from HeLa whole cell lysates with (Lane 2) or without (Lane 1) treatment of sodium butyrate (30 mM, 4 hours) using acetyl-histone H4 (Lys8) rabbit pAb (1:2000).
Scientific DescriptionThe ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code”. Lysine acetylation in core histones occurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation and nuclear import. In most species, histone H2A is primarily acetylated at Lys5, 9, 15 and 36; H2B is primarily acetylated at Lys5, 12, 15, 16 and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56 and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16 and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of histone lysine acetylation status is impaired in cancers and other diseases. Therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugsReferences
  • Liu et al. (2014). Human borna disease virus infection impacts host proteome and histone lysine acetylation in human oligodendroglia cells. Virology 464-465, 196-205. PMID: 25086498
Storage & StabilityThe antibody is delivered in lyophilized powder. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds, and then reconstitute it with the antibody stabilizer provided. The stabilizer contains PBS, 50% glycerol, and 0.01% sodium azide. Please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Antibody is stable for 12 months from date of reconstitution. Equilibrate the antibody to room temperature for 2 minutes and mix by gently pipetting before use.

**Research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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