企业QQ
官方微信
Source/Purification:
This product is produced by immunizing mice with a synthetic acetyl peptide corresponding to residues surrounding Lys139 of human p53. Antibodies are purified by protein G-conjugated agarose followed by
acetylated p53 (Lys139) peptide affinity chromatography.
Recommended Applications:
ELISA, Dot blot, Western blot. Recommended antibody dilution: WB: 1:2000
| NOTE: For WB, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking. Prepare working dilution immediately before use. Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications |
Scientific Description:
Figure A: Dot blotting analysis on indicated amount of peptides using anti-p53K139ac mouse mAb. The list of peptides is included in the table on the right.
Figure B: Western blotting analysis on 30 μg of crude proteins from human MCF-7 whole cell lysates with (right) or without (left) treatment of etoposide (15ug/ml, 24 hours) and (500ng/ml, 24 hours)Trichostatin A using acetyl-p53 (Lys139) mouse mAb (1: 2000).
Figure C: Western blotting analysis on 30 μg of crude proteins from mouse brain and mouse liver whole tissue lysates using acetyl-p53 (Lys139) mouse mAb (1: 2000).
Storage & Stability:
The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn