Product Description
Size: 100 μl
Ab Type: Polyclonal
Reactivity: H, M, R
Immunogen: Acetylated H2AK13ac-KLH
Host: rabbit
Product DescriptionThe rabbit-derived antibody is purified with protein A-conjugated agarose followed by acetylated Histone H2A at Lys13 peptide affinity chromatography. It specifically recognizes Histone H2A with acetylation at Lys13.Source/PurificationThis product is produced by immunizing rabbits with a synthetic acetyl peptide corresponding to residues surrounding Lys13 of human Histone H2A. Antibodies are purified by protein A-conjugated agarose followed by acetylated Histone H2A (Lys13) peptide affinity chromatography.SpecificityH2ARecommended ApplicationsELISA, WB; Not tested in other applications*WB=Western blotting;Recommended antibody dilutionWB: 1:2000Important
For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking. Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. A. Dot blotting analysis on indicated amount of acetylated H2A peptide at Lys13 (lane 1), acetylated H2A peptide at Lys9 (lane 2), 2ohibutyrylated H2A peptide at Lys9 (lane 3), 3ohbutyrylated H2A peptide at Lys9 (lane 4), succinylated H2A peptide at Lys9 (lane 5), biotinylated H2A peptide at Lys9 (lane 6), acetylated H2A peptide at Lys15 (lane 7), and unmodified H2A peptide at Lys13 (lane 8) using acetyl-Histone H2A (Lys13) rabbit pAb. B. Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (lane 2) or without (lane 1) treatment of sodium butyrate (30 mM, 4 hours) using acetyl-Histone H2A (Lys13) rabbit pAb (1:2000). C. Western blotting analysis on 30 μg of crude proteins from mouse spleen, mouse liver tissue lysate using acetyl-Histone H2A (Lys13) rabbit pAb (1: 1000).Scientific DescriptionThe ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine acetylation in multiple sites, termed as “histone code” Lysine acetylation in core histone occurs in response to various stimuli and plays vital roles in the regulation of many cellular processes including chromatin dynamics, DNA transcription, cell cycle progression, apoptosis, differentiation, and nuclear import.. In most species, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16,and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56,and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. More than 20 histone acetyltransferases (HATs) and 18 histone deacetylases (HDACs) have been identified to date, while the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The regulation of histone lysine acetylation status is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine acetylation have become promising targets for anti-cancer drugs.
Storage & StabilityThe antibody is delivered in lyophilized powder. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds, and then reconstitute it with the antibody stabilizer provided. The stabilizer contains PBS, 50% glycerol, and 0.01% sodium azide. Please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Antibody is stable for 12 months from date of reconstitution. Equilibrate the antibody to room temperature for 2 minutes and mix by gently pipetting before use.** Research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.