Ptmbiolabs/Pan anti-acetyllysine antibody (clone Kac-11)/100 μl/PTM-102

Product DescriptionSize: 100 μlAb Type: MonoclonalReactivity: AllImmunogen: Acetylated BSAHost: Mouse

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Product DescriptionThe mouse monoclonal antibody is produced by immunizing animals with acetylated BSA. Antibodies are purified from hybridoma cells by using acetyllysine-conjugated agarose.SpecificityAnti-acetyllysine antibody detects proteins which are post-translationally modified with acetylation at their lysine residues. The antibody recognizes acetylated lysine in a wide range of sequence contexts. The antibody has been shown to react with as little as 4 ng of chemically acetylated BSA while not cross-reacting with 500 ng of non-acetylated BSA and other modified BSA.Recommended ApplicationsELISA, WB, IF, IPWB=Western blotting; IF=Immunofluorescence; IP=Immunoprecipitation.Recommended DilutionWB:1:1000, IF: 1:500; IP: 1:200.IMPORTANT NOTESFor Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1x TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight.

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Scientific DescriptionThe reversible post-translational ε-amino lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin structure, gene expression, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The mis-regulated protein acetylation status has been implicated in cancers and other diseases, and HDACs have become likely targets for anti-cancer drugs.References

• He, Dongli, et al. “Global Proteome Analyses of Lysine Acetylation and Succinylation Reveal the Widespread Involvement of both Modification in Metabolism in the Embryo of Germinating Rice Seed.” Journal of Proteome Research 15.3 (2016): 879-890.PMID: 26767346
• Nowak, Grazyna, and Judit Megyesi. “Protein kinase Cα mediates recovery of renal and mitochondrial functions following acute injury.” FEBS Journal (2019).PMID: 31659858

Storage & StabilityThe antibody is delivered in lyophilized powder. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds, and then reconstitute it with the antibody stabilizer provided. The stabilizer contains PBS, 50% glycerol, and 0.01% sodium azide. Please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Antibody is stable for 12 months from date of reconstitution. Equilibrate the antibody to room temperature for 2 minutes and mix by gently pipetting before use.**Research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.